Description
Humana Press Inc. The Elisa Guidebook by John R. Crowther
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research._x000D_ _x000D_
Overview of ELISA in Relation to Other Disciplines_x000D_
Systems in ELISA_x000D_
Stages in ELISA_x000D_
Titration of Reagents_x000D_
Theoretical Considerations_x000D_
Practical Exercises_x000D_
Monoclonal Antibodies_x000D_
Validation of Diagnostic Tests for Infectious Diseases_x000D_
Charting Methods for Internal Quality Control_x000D_
Immunochemical Techniques_x000D_
Test Questions_x000D_