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Therapeutic Applications Of Rnai Methods And Protocols (Methods In Molecular Biology Volume 555) 2009 Edition at Meripustak

Therapeutic Applications Of Rnai Methods And Protocols (Methods In Molecular Biology Volume 555) 2009 Edition by OLSON-REIDHAAR ET.AL, HUMANA PRESS

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    PublisherHUMANA PRESS
    Publish YearJuly 2009


    HUMANA PRESS Therapeutic Applications Of Rnai Methods And Protocols (Methods In Molecular Biology Volume 555) 2009 Edition by OLSON-REIDHAAR ET.AL

    The phenomenon of RNA interference has rapidly moved from groundbreaking scientific discovery to promising therapeutic approach. However, even as RNAi-based drugs enter the clinic, significant challenges remain, particulary in the area of delivery. Therapeutic Applications of RNAi provides detailed protocols in key areas of current focus, including testing of delivery vehicles, identification of appropriate model systems, and evaluation of the effects of RNAi in vivo. Produced by a team of internationally renowned authors, the volume describes the therapeutic applications of RNAi and potential pitfalls in oncology, viral infections and CNS disease, using a variety of delivery methods, including liposomes, peptide-based nanoparticles, polycationic polymers, and viral vehicles. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Therapeutic Applications of RNAi is an ideal guide for scientists attempting to solve the numerous challenges in this field and revolutionize the treatment of disease. 1. Development and Application of a Dual-Purpose Nanoparticle Platform for Delivery and Imaging of siRNA in TumorsZdravka Medarova, Mohanraja Kumar, Shu-wing Ng, and Anna Moore2. Targeted Gene Silencing into Solid Tumors with Electrically Mediated siRNA DeliveryMuriel Golzio, Laurent Mazzolini, Aurelie Paganin-Gioanni, and Justin Teissie3. Liposomal siRNA for Ovarian CancerLingegowda S. Mangala, Hee Dong Han, Gabriel Lopez-Berestein, and Anil K. Sood4. Therapeutic Applications of RNAi for Silencing Virus ReplicationRalph A. Tripp and Stephen Mark Tompkins5. RNAi in the Malaria Vector, Anopheles gambiaeFlaminia Catteruccia and Elena A. Levashina6. RNAi Using a Chitosan/siRNA Nanoparticle System: in vitro and in vivo ApplicationsMorten Ostergaard Andersen, Kenneth Alan Howard, and Jorgen Kjems7. Lentiviral and Adeno-Associated Vector-Based Therapy for Motor Neuron Disease through RNAiChris Towne and Patrick Aebischer8. Studying Autoimmunity by in vivo RNA InterferenceStephan Kissler9. Transcriptional Gene Silencing Using Small RNAsDaniel H. Kim and John J. Rossi10. Alternative Splicing as a Therapeutic Target for Human DiseasesKenneth J. Dery, Veronica Gusti, Shikha Gaur, John E. Shively, Yun Yen, and Rajesh K. Gaur