Description
COLD SPRING HARBOR LABORATORY PRESS Transcriptional Regulation In Eukaryotes Concepts Strategies And Techniques 2Nd Edition 2009 Edition by CAREY,M.F. & SMALE,S.T.
The first edition of the highly successful Transcriptional Regulation in Eukaryotes, written by Michael Carey and Stephen Smale at UCLA, provided a comprehensive source of strategic, conceptual, and technical information for investigating the complexities of gene regulation at the level of transcription. With the ever-increasing importance of genome data and the appearance of new and better techniques, the second edition of this book has added a third author, Craig Peterson at the University of Massachusetts Medical School. In addition to a new chapter on the in vitro analysis of chromatin templates for DNA-binding studies and transcription, this second edition has been extensively rewritten and updated to discuss new advances in the field and their impact on gene regulation mechanisms. The second edition retains the approach of the first in covering both the conceptual and practical aspects of how to study the regulation of a newly isolated gene and the biochemistry of a new transcription factor. Transcriptional Regulation in Eukaryotes serves as both a powerful textbook and manual for advanced instruction in molecular biology which -supplements clearly written text with extensive illustrations-puts methods in the context of underlying theory-gives expert recommendations on experimental strategies-encourages creativity in investigative design-explains protocols for essential techniques step by step, with extensive advice on troubleshooting-provides the latest methods in use in the field This important and unique book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states, providing underlying theory and perspective to the newcomer and the latest techniques to the expert. Part 1 Foodborne pathogen surveillance and outbreak investigation: Surveillance for foodborne pathogens in humans; Systems for real-time, linked foodborne pathogen surveillance; Detection, investigation and control of outbreaks of foodborne disease; Attributing the burden of foodborne disease to specific sources of infection; Determining the economic costs and global burden of foodborne disease. Part 2 Subtyping of foodborne pathogens: Phenoytypic subtyping; Pulsed-field gel electrophoresis and other commonly used molecular methods; Emerging bacterial subtyping methods; Development, validation and quality assurance of subtyping methods. Part 3 Molecular methods, genomics and other emerging approaches in the surveillance and study of foodborne pathogens: Sample preparation for detection by molecular biological methods; A comparison of molecular technologies and genomotyping for tracing and strain characterisation of Campylobacter isolates; Investigating foodborne pathogens using comparative genomics; Protein-based analysis and other new and emerging non-nucleic acid based methods; Virulotyping; Using ribotyping to trace foodborne aerobic sporeforming bacteria in the factory: A case study; Biotracing: A novel concept in food safety integrating microbiology knowledge, complex systems approaches and probabilistic modeling. Part 4 Tracing pathogens in particular food chains: Red meat and game production chains and the abattoir; Fish production chains; Poultry and egg production chains; Dairy production; Molluscan shellfish production chains; Fruit and vegetable production chains.