Description
Humana Viral Vectors For Gene Therapy Methods And Protocols by Curtis A. Machida
Viral Vectors for Gene Therapy: Methods and Protocols consists of 30 ch- ters detailing the use of herpes viruses, adenoviruses, adeno-associated viruses, simple and complex retroviruses, including lentiviruses, and other virus systems for vector development and gene transfer. Chapter cont- butions provide perspective in the use of viral vectors for applications in the brain and in the central nervous system. Viral Vectors for Gene Therapy: Methods and Protocols contains step-by-step methods for successful rep- cation of experimental procedures, and should prove useful for both experienced investigators and newcomers in the field, including those beginning graduate study or undergoing postdoctoral training. The "Notes" section contained in each chapter provides valuable troublesho- ing guides to help develop working protocols for your laboratory. With Viral Vectors for Gene Therapy: Methods and Protocols, it has been my intent to develop a comprehensive collection of modern molecular methods for the construction, development, and use of viral vectors for gene transfer and gene therapy. I would like to thank the many chapter authors for their contributions. They are all experts in various aspects of viral vectors, and I appreciate their efforts and hard work in developing comprehensive chapters. As editor, it has been a privilege to preview the development of Viral Vectors for Gene Therapy: Methods and Protocols, and to acquire insight into the various methodological approaches from the many different contri- tors. Use of the Herpes Simplex Viral Genome to Construct Gene Therapy VectorsEdward A. Burton, Shaohua Huang, William F. Goins, and Joseph C. GloriosoConstruction of Multiply Disabled Herpes Simplex Viral Vectors for Gene Delivery to the Nervous SystemCaroline E. Lilley and Robert S. CoffinImproved HSV-1 Amplicon Packaging System Using ICP27-Deleted, Oversized HSV-1 BAC DNAYoshinaga Saeki, Xandra O. Breakefield, and E. Antonio ChioccaHerpes Simplex Amplicon VectorsCharles J. Link, Nicholas N. Vahanian, and Suming WangStrategies to Adapt Adenoviral Vectors for Targeted DeliveryCatherine R. O'Riordan, Antonius Song, and Julia LanciottiUse of Recombinant Adenovirus for Gene Transfer into the Rat Brain: Evaluation of Gene Transfer Efficiency, Toxicity, and Inflammatory and Immune ReactionsAndres Hurtado-Lorenzo, Anne David, Clare Thomas, Maria G. Castro, and Pedro R. LowensteinGeneration of Adenovirus Vectors Devoid of All Virus Genes by Recombination Between Inverted RepeatsHartmut Stecher, Cheryl A. Carlson, Dmitry M. Shayakhmetov, and Andre LieberPackaging Cell Lines for Generating Replication-Defective and Gutted Adenoviral VectorsJeffrey S. Chamberlain, Catherine Barjot, and Jeannine ScottImproving the Transcriptional Regulation of Genes Delivered by Adenovirus VectorsSemyon Rubinchik, Jan Woraratanadharm, Jennifer Schepp, and Jian-yun DongTargeted Integration by Adeno-Associated VirusMatthew D. Weitzman, Samuel M. Young, Jr., Toni Cathomen, and Richard Jude SamulskiDevelopment and Optimization of Adeno-Associated Virus Vector Transfer into the Central Nervous SystemMatthew J. During, Deborah Young, Kristin Baer, Patricia Lawlor, and Matthias KlugmannA Method for Helper Virus-Free Production of Adeno-Associated Virus VectorsRoy F. Collaco and James P.TrempeNovel Tools for Production and Purification of Recombinant Adeno-Associated Viral VectorsJulian D. Harris, Stuart G. Beattie, and J. George DicksonRecombinant Adeno-Associated Viral Vector Types 4 and 5: Preparation and Application for CNS Gene TransferBeverly L. Davidson and John A. ChioriniTrans-Splicing Vectors Expand the Packaging Limits of Adeno-Associated Virus for Gene Therapy ApplicationsDongsheng Duan, Yongping Yue, Ziying Yan, and John F. EngelhardtGeneration of Retroviral Packaging and Producer Cell Lines for Large-Scale Vector Production with Improved Safety and TiterThomas W. Dubensky, Jr. and Sybille L. SauterAn Ecdysone-Inducible Expression System for Use with RetrovirusesKaren Morse and John OlsenIn Vivo Infection of Mice by Replication-Competent MLV-Based Retroviral VectorsEstanislao Bachrach, Mogens Duch, Mireia Pelegrin, Hanna Dreja, Finn Skou Pedersen, and Marc PiechaczykDevelopment of Simian Retroviral Vectors for Gene DeliveryBiao Li and Curtis A. MachidaSelf-Inactivating Lentiviral Vectors and a Sensitive Cre-loxP Reporter SystemLung-Ji Chang and Anne-Kathrin ZaissLentiviral Vectors for Gene Transfer to the Central Nervous System: Applications in Lysosomal Storage Disease Animal ModelsDeborah J. Watson and John H. WolfeA Highly Efficient Gene Delivery System Derived from Feline Immunodeficiency Virus (FIV)Sybille L. Sauter, Medhi Gasmi, and Thomas W. Dubensky, JrA Multigene Lentiviral Vector System Based on Differential SplicingYonghong Zhu and Vicente PlanellesProduction of Trans-Lentiviral Vector with Predictable SafetyJohn C. Kappes, Xiaoyun Wu, and John K. WakefieldHuman Immunodeficiency Virus Type 1-Based Vectors for Gene Delivery to Human Hematopoietic Stem CellsAli Ramezani and Robert G. Hawley