Description
Humana Wnt Signaling Methods and Protocols by Quinn Barrett, Lawrence Lum
Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. _x000D_Practical and authoritative, Wnt Signaling: Methods and Protocols aims to support researchers in understanding Wnt signal transduction in any effort to improve outcomes in regenerative medicine and cancer management._x000D_ Table of contents : - _x000D_
1. Visualizing Wnt Palmiteoylation in Single Cells_x000D_
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Xinxin Gao and Rami N. Hannoush_x000D_
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2. Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition Reaction_x000D_
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Rubina Tuladhar, Nageswari Yarravarapu, and Lawrence Lum_x000D_
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3. Biochemical Methods to Analyze Wnt Protein Secretion_x000D_
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Kathrin Glaeser, Michael Boutros, and Julia Christina Gross_x000D_
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4. Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum_x000D_
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Xinjun Zhang and Xi He_x000D_
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5. Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway_x000D_
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Xiaomo Jiang and Feng Cong_x000D_
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6. A Simple Method to Assess Abundance of the b-Catenin Signaling Pool in Cells_x000D_
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Annette S. Flozak, Anna P. Lam, and Cara J. Gottardi_x000D_
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7. Wnt-Dependent Control of Cell Polarity in Cultured Cells_x000D_
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Kristin B. Runkle and Eric S. Witze_x000D_
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8. The Use of Chick Embryos to Study Wnt Signaling/Gradients_x000D_
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Lisa M. Galli, Tiffany Barnes, and Laura W. Burrus9. Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors_x000D_
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Nicola Facchinello, Marco Schiavone, Andrea Vettori, Francesco Argenton, and Natascia Tiso_x000D_
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10. Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo_x000D_
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Jesung Moon and James F. Amatruda_x000D_
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11. Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extracts_x000D_
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Annastasia Simone Hyde, Brian I. Hang, and Ethan Lee_x000D_
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12. Delivery of the Porcupine Inhibitor WNT974 in Mice_x000D_
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Li-shu Zhang and Lawrence Lum_x000D_
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13. Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in Osteogenesis_x000D_
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Zhendong A. Zhong, Nicole J. Ethen, and Bart O. Williams_x000D_
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14. Monitoring Wnt/b-Catenin Signaling in Skin_x000D_
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Amy Ku, Qi Miao, and Hoang Nguyen_x000D_
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<15. The Generation of Organoids for Studying Wnt Signaling_x000D_
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Jarno Drost, Benedetta Artegiani, and Hans Clevers16. Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells_x000D_
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Ian Huggins, David Brafman, and Karl Willert_x000D_
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17. Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions_x000D_
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Xiaoping Bao, Xiaojun Lian, and Sean P. Palecek_x000D_