Description
Springer-Verlag New York Inc. Applications Of Mass Spectrometry In Life Safety by Crisan Popescu , Alina D. Zamfir , Nicolae Dinca
Mass Spectrometry (Ms) Along With Its Hyphenated Techniques Is Capable Of High Throughput Sensitivity Accuracy And Selectivity For The Analysis Of Structure And Composition Of Almost Any Product. Like In Electrophoresis Ms Separates Mo- Cules Based On The Mass-To-Charge Ratio. In Case Of Gel Electrophoresis (Sds- Page) A Well-Known And Efficient Bioanalytical Technique Proteins Bear Negative Charges But Have The Same Charge Density So Proteins Are Separated According To Their Size. Similarly In Case Of Ms Analysis Proteins Carry The Same Charge And Are Separated By Their Molecular Weight. Unlike Sds-Page However Modern Ultra High Resolution Ms Discerns Very Small Mass Differences And Can Resolve And Completely Identify In A Single Experiment Species Of The Same Nominal Mass In Complex Biological Mixtures. Consequently Ms Can Be Used For The Structural Characterization Identification And Sensitive Detection Of Mixtures Of Biomolecules Or For Assessing The Quality Of Isolated Proteins (Purity Integrity Or Post-Translational Modifications For Example) Carbohydrates Nucleic Acids Drugs Metabolites Pollutants Etc. In The Post-Genome Era Ms Is Continuously Developing As One Of The Most Re- Able Analytical Method For Elucidating The Structure Of Molecules Originating From Various Biological Matrices. The Potential Of Ms For High-Sensitive Structural A- Lyses Became Unsurpassable After The Introduction Of Electrospray (Esi) And Matrix Assisted Laser/Desorption Ionization (Maldi) Methods On One Hand And The Pos- Bility To Deduce In Detail Unknown Biopolymer Structures By Highly Accurate Mo- Cular Mass Measurement Followed By Sequencing Using Dissociation Techniques Based On Multiple Stage Ms On The Other.