Arabidopsis Protocols 2Nd Edition by Julio Salinas, Jose J. Sanchez-Serrano , Springer

Description

Springer Arabidopsis Protocols 2Nd Edition by Julio Salinas, Jose J. Sanchez-Serrano

For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world. During this time, a huge amount of knowledge has been acquired on the biology of this plant species, which has resulted in the development of molecular tools that account for much more efficient research. The significance that Arabidopsis would attain in biological research may have been difficult to foresee in the 1980s, when its use in the laboratory started. In the meantime, it has become the model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse have for animal systems. Today, it is difficult to envision research at the cutting edge of plant biology without the use of Arabidopsis. Since the first edition of Arabidopsis Protocols appeared, new developments have fostered an impressive advance in plant biology that prompted us to prepare Arabidopsis Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics has encouraged scientists to explore new scenarios that are extending the limits of our knowledge._x000D_ _x000D_Part I. Growing Arabidopsis_x000D_ _x000D_ Growth of Plants and Preservation of Seeds_x000D_ Luz Rivero-Lepinckas, Deborah Crist, and Randy Scholl_x000D_ _x000D_ Prevention and Control of Pests and Diseases_x000D_ Jenifer Bush, Georg Jander, and Frederick M. Ausubel_x000D_ _x000D_ Growth of Other Species Related to Arabidopsis thaliana_x000D_ Krithika Yogeeswaran and Mikhail Elia Nasrallah. Grafting, Katherine Bainbridge, Tom Bennett, Colin Turnbull, and Ottoline Leyser_x000D_ _x000D_ Synchronization, Transformation, and Cryopreservation of Suspension-Cultured Cells_x000D_ Margit Menges and James A. H. Murray_x000D_ _x000D_ Part II. Genetic Analyses_x000D_ _x000D_ Genetic Analysis_x000D_ Maarten Koornneef, Carlos Alonso-Blanco, and Piet Stam_x000D_ _x000D_ QTL Analysis_x000D_ Carlos Alonso-Blanco, Maarten Koornneef, and Johan W. van Ooijen_x000D_ _x000D_ EMS Mutagenesis of Arabidopsis_x000D_ YongSig Kim, Karen S. Schumaker, and Jian-Kang Zhu_x000D_ _x000D_ Low-Resolution Mapping of Untagged Mutations_x000D_ M. Rosa Ponce, Pedro Robles, Francisca M. Lozano, M. Asuncion Brotons, and Jose L. Micol_x000D_ _x000D_ Gene Identification and Cloning by Molecular Marker Mapping_x000D_ Georg Jander_x000D_ _x000D_ High-Throughput TILLING for Arabidopsis_x000D_ Bradley J. Till, Trenton Colbert, Christine Codomo, Linda Enns, Jessica Johnson, Steven H. Reynolds, Jorja G. Henikoff, Elizabeth A. Greene, Michael N. Steine, Luca Comai, and Steven Henikoff_x000D_ _x000D_ Genotyping and Mapping With High-Density Oligonucleotide Arrays_x000D_ Justin Borevitz_x000D_ _x000D_ Forward Genetic Screening of Insertional Mutants_x000D_ Anja Schneider and Dario Leister_x000D_ _x000D_ PCR-Based Screening for Insertional Mutants_x000D_ Anna N. Stepanova and Jose M. Alonso_x000D_ _x000D_ Cytogenetic Analysis of Arabidopsis_x000D_ Martin Lysak, Paul Fransz, and Ingo Schubert_x000D_ _x000D_ Using Information From Public Arabidopsis Databases to Aid Research_x000D_ Margarita Garcia-Hernandez and Leonore Reiser_x000D_ _x000D_ Part III. Transient and Stable Transformation_x000D_ _x000D_ In PlantaAgrobacterium-Mediated Transformation by Vacuum Infiltration_x000D_ Brian W. Tague and Joanna Mantis_x000D_ _x000D_ Transient Expression Assay by Agroinfiltration of Leaves_x000D_ Min Woo Lee and Yinong Yang_x000D_ _x000D_ Functional Analysis of Transcription Factors by Microparticle Bombardment_x000D_ Ida Ruberti, Giovanna Sessa, and Giorgio Morelli_x000D_ _x000D_ Transfection Assays With Protoplasts Containing Integrated Reporter Genes_x000D_ Shiv Tiwari, Shucai Wang, Gretchen Hagen, and Tom J. Guilfoyle_x000D_ _x000D_ Chloroplast Genetic Engineering Via Organogenesis or Somatic Embryogenesis_x000D_ Amit Dhingra and Henry Daniell_x000D_ _x000D_ b-Glucuronidase as Reporter Gene: Advantages and Limitations_x000D_ Kye-Won Kim, Vincent R. Franceschi, Laurence B. Davin, and Norman G. Lewis_x000D_ _x000D_ Use of Fluorescent Proteins as Reporters_x000D_ Guido Jach_x000D_ _x000D_ Luciferases as Reporter Genes_x000D_ Megan M. Southern, Paul E. Brown, and Anthony Hall_x000D_ _x000D_ Aequorin as a Reporter Gene_x000D_ Christoph Plieth_x000D_ _x000D_ Applications of Chemical-Inducible Expression Systems in Functional Genomics and Biotechnology_x000D_ Jianru Zuo, Peter D. Hare, and Nam-Hai Chua_x000D_ _x000D_ Part IV. Transcriptomics_x000D_ _x000D_ RNA Extraction_x000D_ Huazhong Shi and Ray Bressan_x000D_ _x000D_ Target Preparation for DNA Microarray Hybridization_x000D_ Tong Zhu, Sherman H. Chang, and Pedro Gil_x000D_ _x000D_ Statistical Issues in Microarray Data Analysis_x000D_ Willem A. Rensink and Samuel P. Hazen_x000D_ _x000D_ Preparation and Quality Assesment of RNA From Cell-Specific Samples Obtained by Laser Microdissection_x000D_ Regine Kleber and Julia Kehr_x000D_ _x000D_ Part V. Proteomics_x000D_ _x000D_ Protein Isolation and Second-Dimension Electrophoretic Separation_x000D_ Bernhard Schlesier and Hans-Peter Mock_x000D_ _x000D_ Isolation of Nuclear Proteins_x000D_ Tomasz T. Calikowski and Iris Meier_x000D_ _x000D_ Purification and Fractionation of Membranes for Proteomic Analysis_x000D_ Anne Marmagne, Daniel Salvi, Norbert Rolland, Genevieve Ephritikhine, Jacques_x000D_