Description
CBS Publishers & Distributors Biochemistry For Medical Laboratory Technology Students by Harbans Lal and Ashuma Sachdeva
Biochemistry for Medical Laboratory Technology students has been organized as per recent curriculum of the Bsc MLT course,
being followed in most of the universities. The complete syllabus has been divided in to three parts, comprising of 54 chapters. All
the chapters place emphasis on the clinical relevance of biochemistry. For better understanding of the subject, wherever desired,
the text has been supplemented with suitable diagrams and tables to make it even more lucid for the students.
• PART I Includes Basic Principles of Biochemistry. This section deals with an Introduction to MLT; Chemistry of
macromolecules ; safety measures ; First aid ; storage and handling of chemicals ; Units ; Reference range ;Statistics ;
Radioisotopes ;Biophysical chemistry; Glass and electric apparatus ;collection and handling of specimen and disposal of waste
;Preparation of distilled water ; reagents and solutions , and buffers ; Estimation of various analytes ; and urine and stone analysis ,
which are of prime importance for a medical laboratory Technologist.
• PART II Includes Analytical Biochemistry and Metabolism. This section deals with Enzymes and vitamins; Metabolism of
macromolecules and hemoglobin; and some of the techniques like Chromatography and Electrophoresis.
• PART III Includes Clinical Biochemistry. This section deals with quality control ; Automation in clinical chemistry and
laboratory organization ; organ function tests ; Minerals ; hormones ; Special Investigations like diabetic and cardiac makers , etc.;
Biochemical analysis and biological fluids and stool.
All the three parts also have relevant practical exercises. Further, some important questions, which a student is expected to know
after understanding the subject, have been included at the end of each chapter.
A lot of effort has gone into making this book simple and enjoyable to read and retain for a student. We hope that the students will be
benefitted immensely by this comprehensive volume.
Tale of Contents:-
PART I
Basic Principles of Biochemistry
Chapter 1 Introduction to Medical Laboratory Technology 3
Chapter 2 Carbohydrates 6
Chapter 3 Lipids 15
Chapter 4 Proteins and Amino Acids 22
Chapter 5 Digestion and Absorption of Carbohydrates, Lipids and Proteins 34
Chapter 6 Safety Measures in a Clinical Chemistry Laboratory 38
Chapter 7 Storage and Handling of Laboratory Chemicals 39
Chapter 8 First Aid and Emergency Treatment in a Clinical Chemistry Laboratory 45
Chapter 9 Units of Measurements 49
Chapter 10 Radioisotopes and Radiation Hazards 53
Chapter 11 Biophysical Chemistry 56
Chapter 12 Reference Range 61
Chapter 13 Laboratory Glass Apparatus 64
Chapter 14 Electrical Apparatus 77
Chapter 15 Collection and Handling of Blood Specimen 82
Chapter 16 Infectious Biological Material 92
Chapter 17 Disposal of Biological Waste 96
Chapter 18 Distilled Water 99
Chapter 19 Reagents and Solutions 102
Chapter 20 PH, Buffers and Indicators 106
Chapter 21 Qualitative Analysis of Urine 112
Chapter 22 Colorimetry 121
Chapter 23 Routine Clinical Chemistry Investigations of Blood 126
Chapter 24 Electrolytes 137
Chapter 25 Statistical Methods 142
Chapter 26 Biochemical Analysis of Kidney and Gallbladder Stones 146
PART II
Analytical Biochemistry and Metabolism
Chapter 27 Enzymes 151
Chapter 28 Vitamins and Coenzymes 159
Chapter 29 Metabolism of Carbohydrates 172
Chapter 30 Metabolism of Lipids 184
Chapter 31 Metabolism of Proteins and Amino Acids 195
Chapter 32 Nucleic Acids 208
Chapter 33 Metabolism of Hemoglobin 214
Chapter 34 Lipid Profile 218
Chapter 35 Estimation of Serum Enzymes 223
Chapter 36 Spectroscopy 227
Chapter 37 Chromatography 229
Chapter 38 Electrophoresis 236
PART III
Clinical Biochemistry
Chapter 39 Enzyme Linked Immunosorbent Assay 243
Chapter 40 Quality Control 247
Chapter 41 Automation in Clinical Chemistry Laboratory 254
Chapter 42 Organization of a Clinical Chemistry Laboratory 262
Chapter 43 Pancreatic Function Tests 266
Chapter 44 Liver Function Tests 268
Chapter 45 Gastric Function Tests 273
Chapter 46 Kidney Function Tests 277
Chapter 47 Thyroid Function Tests 287
Chapter 48 Quantitative Estimation of Some Biochemical Constituents in Urine 291
Chapter 49 Minerals 296
Chapter 50 Hormone Profiles 304
Chapter 51 Special Biochemical Investigations 308
Chapter 52 Biochemical Analysis of CSF 314
Chapter 53 Biochemical Analysis of Ascitic and Pleural Fluids 316
Chapter 54 Biochemical Examination of Stool 318
Index