Biophysics Tools And Techniques by Mark C. Leake, Taylor & Francis

Description

Taylor & Francis Biophysics Tools And Techniques by Mark C. Leake

An Up-to-Date Toolbox for Probing Biology_x000D__x000D__x000D_Biophysics: Tools and Techniques covers the experimental and theoretical tools and techniques of biophysics. It addresses the purpose, science, and application of all physical science instrumentation and analysis methods used in current research labs._x000D__x000D__x000D__x000D__x000D__x000D_The book first presents the historical background, concepts, and motivation for using a physical science toolbox to understand biology. It then familiarizes undergraduate students from the physical sciences with essential biological knowledge. _x000D__x000D__x000D__x000D__x000D__x000D_The text subsequently focuses on experimental biophysical techniques that primarily detect biological components or measure/control biological forces. The author describes the science and application of key tools used in imaging, detection, general quantitation, and biomolecular interaction studies, which span multiple length and time scales of biological processes both in the test tube and in the living organism._x000D__x000D__x000D__x000D__x000D__x000D_Moving on to theoretical biophysics tools, the book presents computational and analytical mathematical methods for tackling challenging biological questions including exam-style questions at the end of each chapter as well as step-by-step solved exercises. It concludes with a discussion of the future of this exciting field._x000D__x000D__x000D__x000D__x000D__x000D_Future innovators will need to be trained in multidisciplinary science to be successful in industry, academia, and government support agencies. Addressing this challenge, this textbook educates future leaders on the development and application of novel physical science approaches to solve complex problems linked to biological questions._x000D__x000D__x000D_Features:_x000D__x000D__x000D__x000D__x000D_Provides the full, modern physical science toolbox of experimental and analytical techniques, such as bulk ensemble methods, single-molecule tools, and live-cell and test tube methods_x000D_Incorporates worked examples for the most popular physical science tools, including full diagrams and a summary of the science involved in the application of the tool_x000D_Reinforces the understanding of key concepts and biological questions _x000D__x000D__x000D_A solutions manual is available upon qualifying course adoption._x000D_ _x000D_ Introduction_x000D_ The motivation for biophysics_x000D_ What do we mean by a "toolbox?"_x000D_ Makeup of the subsequent chapters in this book_x000D_ Once more, unto the breach_x000D_ Summary points _x000D_ Questions _x000D_ References_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Orientation for the bio-curious_x000D_ Introduction: the material stuff of life _x000D_ Architecture of organisms, tissues and cells, and the bits in between_x000D_ The chemicals that make cells work_x000D_ Cell processes_x000D_ Physical quantities in biology_x000D_ Summary points_x000D_ Questions_x000D_ References_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Making light work in biology_x000D_ Introduction_x000D_ Basic UV-VIS-IR absorption, emission, and elastic light scattering methods_x000D_ Light microscopy basics_x000D_ Non-fluorescence microscopy _x000D_ Fluorescence microscopy: the basics_x000D_ Basic fluorescence microscopy illumination modes_x000D_ Summary points_x000D_ Questions_x000D_ References_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Making light work harder in biology_x000D_ Introduction_x000D_ Super-resolution microscopy_x000D_ Foerster resonance energy transfer (FRET)_x000D_ Fluorescence correlation spectroscopy (FCS)_x000D_ Light microscopy of deep samples_x000D_ Advanced biophysical techniques using elastic light scattering_x000D_ Tools using the inelastic scattering of light_x000D_ Summary points_x000D_ Questions_x000D_ References_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Detection and imaging tools that use non-optical waves_x000D_ Introduction_x000D_ Electron microscopy (EM)_x000D_ X-ray tools_x000D_ NMR and other radio frequency and microwave resonance spectroscopies_x000D_ Tools that use gamma rays, radioisotope decay, and neutrons_x000D_ Summary points_x000D_ Questions_x000D_ References_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Forces_x000D_ Introduction_x000D_ Rheology and hydrodynamics tools_x000D_ Optical force tools_x000D_ Magnetic force methods_x000D_ Scanning probe microscopy and force spectroscopy_x000D_ Electrical force tools_x000D_ Tools to mechanically probe cells and tissues_x000D_ Summary points_x000D_ Questions_x000D_ References_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Complementary experimental tools_x000D_ Introduction_x000D_ Bioconjugation_x000D_ Model organisms_x000D_ Molecular cloning_x000D_ Making crystals_x000D_ High-throughput techniques_x000D_ Characterizing physical properties of biological samples in bulk_x000D_ Biomedical physics tools_x000D_ Summary points_x000D_ Questions_x000D_ References_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Theoretical biophysics_x000D_ Introduction_x000D_ Molecular simulation methods_x000D_ Mechanics of biopolymers_x000D_ Reaction, diffusion, and flow _x000D_ Advanced in silico analysis tools _x000D_ Rigid-body mechanics_x000D_ Summary points_x000D_ Questions_x000D_ References_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Emerging biophysics techniques_x000D_ Introduction_x000D_ Systems biology: "systems biophysics"_x000D_ Synthetic biology and bionanotechnology_x000D_ Personalizing healthcare_x000D_ Extending length and time scales to quantum and ecological biophysics_x000D_ Summary points_x000D_ Questions_x000D_ References_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Index_x000D_