Description
Humana Press Cell Imaging Techniques 1st Editon 2010 Softbound by Douglas J. Taatjes, Brooke T. Mossman
A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy. Molecular Beacons.- Second-Harmonic Imaging of Collagen.- Visualizing Calcium Signaling in Cells by Digitized Wide-Field and Confocal Fluorescent Microscopy.- Multifluorescence Labeling Techniques and Confocal Laser Scanning Microscopy on Lung Tissue.- Evaluation of Confocal Microscopy System Performance.- Quantitative Analysis of Atherosclerotic Lesion Composition in Mice.- Applications of Microscopy to Genetic Therapy of Cystic Fibrosis and Other Human Diseases.- Laser Scanning Cytometry.- Near-Clinical Applications of Laser Scanning Cytometry.- Laser Capture Microdissection.- Analysis of Asbestos-Induced Gene Expression Changes in Bronchiolar Epithelial Cells Using Laser Capture Microdissection and Quantitative Reverse Transcriptase-Polymerase Chain Reaction.- New Approaches to Fluorescence In Situ Hybridization.- Microarray Image Scanning.- Near-Field Scanning Optical Microscopy in Cell Biology and Cytogenetics.- Porosome.- Secretory Vesicle Swelling by Atomic Force Microscopy.- Imaging and Probing Cell Mechanical Properties With the Atomic Force Microscope.- Reflection Contrast Microscopy.- Three-Dimensional Analysis of Single Particles by Electron Microscopy.- Three-Dimensional Reconstruction of Single Particles in Electron Microscopy.- A New Microbiopsy System Enables Rapid Preparation of Tissue for High-Pressure Freezing.