Description
SPRINGER Cell Migration 2005 Edition by GUAN, JUN-LIN
A collection of classic, novel, and state-of-the-art methods for the study of cell migration in cultured cells, different model organisms, and specialized cells in normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Part I. IntroductionCell Migration: An OverviewDonna J. Webb, Huaye Zhang, and Alan F. HorwitzPart II. Basic Cell Migration and Related AssaysBoyden Chamber AssayHong-Chen ChenWound-Healing AssayLuis G. Rodriguez, Xiaoyang Wu, and Jun-Lin GuanAnalysis of Cell Migration Using the Dunn Chemotaxis Chamber and Time-Lapse MicroscopyClaire M. Wells and Anne J. RidleyCell-Adhesion AssaysDennis F. Kucik and Chuanyue WuCell-Spreading AssaysAllison L. Berrier and Susan E. LaFlammeCell-Scatter AssayHong-Chen ChenCell Migration Analyses Within Fibroblast-Derived 3-D MatricesEdna CukiermanPart III. Analysis of Cell Migration in Specialized Cell SystemsTumor Cell Invasion AssaysLeslie M. ShawAnalysis of Endothelial Cell Migration Under FlowSong LiAngiogenesis Assays in the Chick CAMChris Storgard, David Mikolon, and Dwayne G. StupackInvestigations of Neuronal Migration in the Central Nervous SystemMichael E. Ward and Yi RaoPart IV. Analysis of Cell Migration in Model OrganismsAnalysis of Cell Migration in Caenorhabditis elegansM. Afaq Shakir and Erik A. LundquistAnalysis of Cell Migration Using Drosophila as a Model SystemJocelyn A. McDonald and Denise J. MontellImaging Cell Movement During Dorsal Closure in Drosophila EmbryosWilliam Wood and Antonio JacintoAnalysis of Cell Movements in Zebrafish Embryos: Morphometrics and Measuring Movement of Labeled Cell Populations In VivoDiane S. Sepich and Lilianna Solnica-KrezelThe Xenopus Embryo as a Model System for Studies of Cell MigrationDouglas W. DeSimone, Lance Davidson, Mungo Marsden, and Dominique AlfandariNeural Crest Migration Methods in the Chicken EmbryoMaria Elena de Bellard and Marianne Bronner-FraserIn Vivo and In Vitro Models of Mammalian AngiogenesisMien V. Hoang and Donald R. SengerPart V. Biochemical and Novel Approaches in Cell MigrationMeasurement of Protein Tyrosine Phosphorylation in Cell AdhesionVidhya V. Iyer, Christoph Ballestrem, Joachim Kirchner, Benjamin Geiger, and Michael D. SchallerCell-Adhesion Assays: Fabrication of an E-Cadherin Substratum and Isolation of Lateral and Basal Membrane PatchesFrauke Drees, Amy Reilein, and W. James NelsonApplication of Microscope-Based FRET to Study Molecular Interactions in Focal Adhesions of Live CellsChristoph Ballestrem and Benjamin GeigerUse of Multiphoton Imaging for Studying Cell Migration in the MouseAndrea Flesken-Nikitin, Rebecca M. Williams, Warren R. Zipfel, Watt W. Webb, and Alexander Yu. NikitinUsing Microfluidic Channel Networks to Generate Gradients for Studying Cell MigrationDaniel S. Rhoads, Sharvari M. Nadkarni, Loling Song, Camilla Voeltz, Eberhard Bodenschatz, and Jun-Lin GuanIndex