Description
Humana Chemosensitivity Vol-1 In Vitro Assays by Rosalyn D. Blumenthal
A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity, Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and non-adherent cells for single agents and combinations of agents. In addition to immunohistochemical and imaging approaches, these assays include clonogenic, colorimetric, fluorometric, and physiological assays. Highlights include image analysis to assess drug sensitivity, high throughput approaches using green fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay used in biotechnology. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides protocols for classifying tumors into response categories and customizing chemotherapy regimens to individual patients. Part I. OverviewAn Overview of Chemosensitivity TestingRosalyn D. BlumenthalPart II. In Vitro Measures of ChemosensitivityClonogenic Cell Survival AssayAnupama Munshi, Marvette Hobbs, and Raymond E. MeynHigh-Sensitivity Cytotoxicity Assays for Nonadherent CellsM. Jules MattesSulforhodamine B Assay and ChemosensitivityWieland VoightUse of the Differential Staining Cytotoxicity Assay to Predict ChemosensitivityGertjan J. L. KaspersCollagen Gel Droplet Culture Method to Examine In Vitro ChemosensitivityHisayuki KobayashiThe MTT Assay to Evaluate ChemosensitivityJack D. BurtonHistoculture Drug Response Assay to Monitor ChemoresponseShinji Ohie, Yasuhiro Udagawa, Daisuke Aoki, and Shiro NozawaIn Vitro Testing of Chemosensitivity in Physiological HypoxiaRita Grigoryan, Nino Keshelava, Clarke Anderson, and C. Patrick ReynoldsChemosensitivity Testing Using Microplate Adenosine Triphosphate-Based Luminescence MeasurementsChristian M. Kurbacher and Ian A. CreeHigh-Throughput Technology: Green Fluorescent Protein to Monitor Cell DeathMarylene Fortin, Ann-Muriel Steff, and Patrice HugoDIMSCAN: A Microcomputer Fluorescence-Based Cytotoxicity Assay for Preclinical Testing of Combination ChemotherapyNino Keshelava, Tomasth Frgala, JirD i Krejsa, Ondrej Kalous, and C. Patrick ReynoldsThe ChemoFx (R) Assay: An Ex Vivo Cell Culture Assay for Predicting Anticancer Drug ResponsesRobert L. Ochs, Dennis Burholt, and Paul KornblithEvaluating Response to Antineoplastic Drug Combinations in Tissue Culture ModelsC. Patrick Reynolds and Barry J. MaurerImage Analysis Using the Fluochromasia Assay to Quantify Tumor Drug SensitivityJohn F. Gibbs, Youcef M. Rustum, and Harry K. SlocumImmunohistochemicalDetection of Ornithine Decarboxylase as a Measure of ChemosensitivityUriel BachrachImmunohistochemistry of p53, Bcl-2 and Ki-67 as Predictors of ChemosensitivityMitsuyoshi Itaya, Jiro Yoshimoto, Kuniaki Kojima, and Seiji KawasakiIndex