Chronic Myeloid Leukemia Methods and Protocols by Shaoguang Li, Haojian Zhang , Humana

Description

Humana Chronic Myeloid Leukemia Methods and Protocols by Shaoguang Li, Haojian Zhang

This volume highlights the molecular and cellular methods used in studying Chronic Myeloid Leukimia (CML) pathogenesis and stem cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls._x000D__x000D__x000D__x000D_Authoritative and cutting-edge, Chronic Myeloid Leukemia: Methods and Protocols aims to ensure successful results in the further study of this vital field._x000D_ Table of contents : - _x000D_ 1. Molecular Detection of BCR-ABL in Chronic Myeloid Leukemia_x000D_ _x000D_ _x000D_ _x000D_ Ya-Zhen Qin and Xiao-Jun Huang_x000D_ _x000D_ _x000D_ _x000D_ 2. Induction of Chronic Myeloid Leukemia in Mice_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Haojian Zhang and Shaoguang Li_x000D_ _x000D_ _x000D_ _x000D_ 3. CML Mouse Model Generated from Leukemia Stem Cells_x000D_ _x000D_ _x000D_ _x000D_ Yiguo Hu_x000D_ _x000D_ _x000D_ _x000D_ 4. Immunological Analyses of Leukemia Stem Cells_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Kazuhito Naka and Yoshihiro Takihara_x000D_ _x000D_ _x000D_ _x000D_ 5. Cell Cycle Analysis of CML Stem Cells using Hoechst33342 and Propidium Iodide_x000D_ _x000D_ _x000D_ _x000D_ Ngoc DeSouza, Megan Zhou, and Yi Shan_x000D_ _x000D_ _x000D_ _x000D_ 6. Histological and In Vivo Microscopic Analysis of the Bone Marrow Microenvironment in a Murine Model of Chronic Myelogenous Leukemia_x000D_ _x000D_ _x000D_ _x000D_ Eva S. Weissenberger and Daniela S. Krause_x000D_ _x000D_ _x000D_ _x000D_ 7. The Culture Repopulation Ability (CRA) Assay and Incubation in Low Oxygen to Test Anti-Leukemic Drugs on Imatinib-resistant CML Stem-like Cells_x000D_ _x000D_ _x000D_ Giulia Cheloni and Michele Tanturli_x000D_ _x000D_ _x000D_ _x000D_ 8. Tumor Suppressor Analysis in CML_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Oliver Herrmann and Mirle Schemionek_x000D_ _x000D_ _x000D_ _x000D_ 9. Detecting Autophagy and Autophagy Flux in Chronic Myeloid Leukemia Cells Using a Cyto ID Fluorescence Spectrophotometric Assay_x000D_ _x000D_ _x000D_ _x000D_ Sujuan Guo, Kevin Pridham, and Zhi Sheng_x000D_ _x000D_ _x000D_ _x000D_ 10. DREAM, A Simple Method for DNA Methylation Profiling by High Throughput Sequencing_x000D_ _x000D_ _x000D_ _x000D_ Jaroslav Jelinek and Jozef Madzo_x000D_ _x000D_ _x000D_ _x000D_ 11. ChIP-seq Analysis of Human Chronic Myeloid Leukemia Cells_x000D_ _x000D_ _x000D_ _x000D_ Lars Anders and Zhaodong Li_x000D_ _x000D_ _x000D_ _x000D_ 12. Quantitative Proteomics Analysis of Leukemia Cells_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Sebastian Halbach, Joern Dengjel, and Tilman Brummer_x000D_ _x000D_ _x000D_ _x000D_ 13. A Convenient Cell Culture Model for CML Acquired Resistance through BCR-ABL Mutations_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Zhiqiang Wang and WenYong Chen_x000D_ _x000D_ _x000D_ _x000D_ 14. High Throughput Screening of Tyrosine Kinase Inhibitor Resistant Genes in CML_x000D_ _x000D_ _x000D_ _x000D_ Leyuan Ma, Justine Roderick, Michelle A. Kelliher, and Michael R. Green_x000D_ _x000D_ _x000D_ _x000D_ 15. Biological Analysis of Human CML Stem Cells; Xenograft Model of Chronic Phase Human Chronic Myeloid Leukemia_x000D_ _x000D_ _x000D_ _x000D_ Sheela A. Abraham_x000D_ _x000D_ _x000D_ _x000D_ 16. New Mouse Models to Investigate the Efficacy of Drug Combinations in Human Chronic Myeloid Leukemia _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Hanyang Lin, Adrian Woolfson, and Xiaoyan Jiang_x000D_ _x000D_ _x000D_ _x000D_ 17. miRNome Analysis of CML Cells_x000D_ _x000D_ _x000D_ Yadong Yang, Nan Ding, Xunong Dong, Xiangdong Fang_x000D_ _x000D_ _x000D_ _x000D_ 18. An Integrative Analysis of microRNA and mRNA Profiling in CML Stem Cells_x000D_ _x000D_ _x000D_ _x000D_ Farah Nassar, Rabab El Eit, and Rihab Nasr_x000D_ _x000D_ _x000D_ _x000D_ 19. Single Cell Cytokine Profiling to Investigate Cellular Functional Diversity in Hematopoietic Malignancies_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ Jonathan J. Chen, Minsuk Kwak, and Rong Fan_x000D_