Clinical Applications Of Pcr 2Nd Edition (Methods In Molecular Biology Volume 336) by LO Y. M. DENNIS ET.AL, HUMANA PRESS

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HUMANA PRESS Clinical Applications Of Pcr 2Nd Edition (Methods In Molecular Biology Volume 336) by LO Y. M. DENNIS ET.AL

In this updated second edition, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction( PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis._x000D_ _x000D_ Introduction to the Polymerase Chain Reaction_x000D_ Y. M. Dennis Lo and K. C. Allen Chan_x000D_ _x000D_ Setting Up a Polymerase Chain Reaction Laboratory_x000D_ Y. M. Dennis Lo and K. C. Allen Chan_x000D_ _x000D_ Real-Time Polymerase Chain Reaction and Melting Curve Analysis_x000D_ Robert J. Pryor and Carl T. Wittwer_x000D_ _x000D_ Qualitative and Quantitative Polymerase Chain Reaction-Based Methods for DNA Methylation Analyses_x000D_ Ivy H. N. Wong_x000D_ _x000D_ In-Cell Polymerase Chain Reaction: Strategy and Diagnostic Applications_x000D_ T. Vauvert Hviid_x000D_ _x000D_ Qualitative and Quantitative DNA and RNA Analysis by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry_x000D_ Chunming Ding_x000D_ _x000D_ Analysis of Polymerase Chain Reaction Products by Denaturing High-Performance Liquid Chromatography_x000D_ Ching-Wan Lam_x000D_ _x000D_ Use of Real-Time Polymerase Chain Reaction for the Detection of Fetal Aneuploidies_x000D_ Bernhard Zimmermann, Lisa Levett, Wolfgang Holzgreve, and Sinuhe Hahn_x000D_ _x000D_ Noninvasive Prenatal Diagnosis by Analysis of Fetal DNA in Maternal Plasma_x000D_ Rossa W. K. Chiu and Y. M. Dennis Lo_x000D_ _x000D_ Clinical Applications of Plasma Epstein-Barr Virus DNA Analysis and Protocols for the Quantitative Analysis of the Size of Circulating Epstein-Barr Virus DNA_x000D_ K. C. Allen Chan and Y. M. Dennis Lo_x000D_ _x000D_ Molecular Analysis of Circulating RNA in Plasma_x000D_ Nancy B. Y. Tsui, Enders K. O. Ng, and Y. M. Dennis Lo_x000D_ _x000D_ Molecular Analysis of Mitochondrial DNA Point Mutations by Polymerase Chain Reaction_x000D_ Lee-Jun C. Wong, Bryan R. Cobb, and Tian-Jian Chen_x000D_ _x000D_ Novel Applications of Polymerase Chain Reaction to Urinary Nucleic Acid Analysis_x000D_ Anatoly V. Lichtenstein, Hovsep S. Melkonyan, L. David Tomei, and Samuil R. Umansky_x000D_ _x000D_ Detection and Quantitation of Circulating Plasmodium falciparum DNA by Polymerase Chain Reaction_x000D_ Shira Gal and James S. Wainscoat_x000D_ _x000D_ Molecular Diagnosis of Severe Acute Respiratory Syndrome_x000D_ Enders K. O. Ng and Y. M. Dennis Lo_x000D_ _x000D_ Genomic Sequencing of the Severe Acute Respiratory Syndrome-Coronavirus_x000D_ Stephen S. C. Chim, Rossa W. K. Chiu, and Y. M. Dennis Lo_x000D_ _x000D_ Index_x000D_