Description
ROYAL SOCIETY OF CHEMISTRY Comet Assay In Toxicology Volume 30 by Diana Anderson and Alok Dhawan
Concerns about the adverse effects of chemicals present in the environment have created a need for better systems to assess their potential consequences on human health. One potential solution is the versatile and state-of-the-art Comet assay. Simple, sensitive, rapid and visual, this modern toxicological method allows quantitative and qualitative assessment of DNA damage in single cells. This assay is used in diverse fields ranging from clinical applications, human monitoring and environmental toxicology through to genetic toxicity testing._x000D_This updated and revised edition of The Comet Assay in Toxicology provides the latest information on this important tool. It addresses, in-depth, the different protocols, statistical analyses and applications used worldwide. It also includes the guidelines recommended by the Working Group on Comet Assay. The book begins with a review of the genesis of the assay for those new to the technique and goes on to explain procedures followed to assess different types of DNA damage, various applications of the assay, and guidelines for the conduct of the assay in in vitro and in vivo systems. New chapters written for this edition will provide information on the most contemporary approaches and applications, including in silico approaches, on meta-analysis of data and on the application of the Comet Assay in nanotoxicology._x000D_This book will serve as both a reference and a guide to students as well as investigators in the biomedical, biochemical and pharmaceutical sciences fields._x000D_ Table of Contents:- _x000D_
The Comet Assay: A Versatile Tool for Assessing DNA Damage; Detection of Oxidised DNA Using DNA Repair Enzymes; Microplate-Based Comet Assay; The Use of Higher Plants in the Comet Assay; Methods for Freezing Blood Samples at 80 DegreesC for DNA Damage Analysis in Human Leukocytes; Development and Applications of the Comet-FISH Assay for the Study of DNA Damage and Repair; Detection of DNA Damage in different organs of the Mouse; Clinical Applications of the Comet Assay; Applications of the Comet Assay in Human Biomonitoring; The Comet Assay in Human Biomonitoring; Comet Assays in Dietary Intervention Trials; The Comet Assay for the Evaluation of Genotoxic Exposure in Aquatic Species; The Alkaline Comet Assay in Prognostic Tests for Male Infertility and Assisted Reproductive Technology Outcomes; he Comet Assay in Sperm - Assessing Genotoxins in Male Germ Cells; Assessment of DNA Damage in alternate to animal models; Neutral bacterial comet assay to assess DNA double-strand breaks in Escherichia coli; In silico prediction of DNA Damage using the Comet Assay data; Comet Assay - Protocols and Testing Strategies; Imaging and Image Analysis in the Comet Assay; Statistical Analysis of Comet Assay Data; Meta-Analysis of the Comet Assay Data of Human Monitoring Studies; Application of the Comet Assay in Nanotoxicology_x000D_