Description
SPRINGER Differential Display Methods And Protocols by LIANG
Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an "open"-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms-from bacteria, fungi, insects, fish, plants, to mammals-even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious "cottage-industry" academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis. Part I. MethodologiesGlobal Analysis of Gene Expression by Differential Display: A Mathematical ModelShitao Yang and Peng LiangAutomation of Fluorescent Differential Display With Digital ReadoutJonathan D. Meade, Yong-jig Cho, Jeffrey S. Fisher, Jamie C. Walden, Zhen Guo, and Peng LiangOrdered Differential DisplayMikhail V. Matz and Ella A. MeleshkevitchGeneCalling (R): Transcript Profiling Coupled to a Gene Database QueryRichard A. ShimketsHigh-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCRDana M. Walters and Pierre E. RouviereVertical Arrays: Microarrays of Complex Mixtures of Nucleic AcidsRosana Risques, Gaelle Rondeau, Martin Judex, Michael McClelland, and John WelshAutomated Pattern Ranking in Differential Display Data AnalysisTero Aittokallio, Pekka Ojala, Timo J. Nevalainen, and Olli S. NevalainenLinking cDNA-AFLP-Based Gene Expression Patterns and ESTsLing Qin, Pjotr Prins, and Johannes HelderPart II. ApplicationsDifferentially Expressed Genes Associated With Hepatitis B Virus HBx and MHBs Protein Function in Hepatocellular CarcinomaDae-Ghon KimIdentification of Disease Markers by Differential Display: Prion DiseaseMichael Clinton, Gino Miele, Sunil Nandi, and Derek McBrideSaturation Screening for p53 Target Genes by Digital Fluorescent Differential DisplayYong-Jig Cho, Susanne Stein, Roger S. Jackson II, and Peng LiangIdentification of p53-Regulated Genes by the Method of Differential DisplayYunping Lin, Roger P. Leng, and Samuel BenchimolIdentification by Differential Display of IL-24 Autocrine Loop Activated by Ras OncogenesZhongjia Tan, Mai Wang, and Peng LiangComprehensive Analysis of Ovarian Gene Expression During Ovulation Using Differential DisplayLawrence L.EspeyFunctional Analysis of Nocturnin: A Circadian Clock-Regulated Gene Identified by Differential DisplayJulie E. Baggs and Carla B. GreenIsolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. crispa by Differential DisplayMami Yamazaki and Kazuki SaitoIdentification of Target Genes of a Yeast Transcriptional RepressorBernard Mai and Linda L. BreedenDetection of an mRNA Polymorphism by Differential DisplayShan Liang, S. Paul Rossby, Peng Liang, Richard C. Shelton, D. Hal Manier, Amitabha Chakrabarti, and Fridolin SulserSilencing in Yeast: Identification of Clr4 TargetsSergey V. Ivanov and Alla V. IvanovaIdentification of mRNA Bound to RNA Binding Proteins by Differential DisplayAnne Carr-Schmid, Xinfu Jiao, and Megerditch KiledjianIndex