Description
Humana Epidermal Cells Methods And Procols by Kursad Turksen
A collection of cutting-edge methods to analyze and manipulate epidermal cellprecursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, and for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. These readily reproducible techniques broaden our understanding of the biology of epidermal cells and of their utility in normal tissue homeostasis and regenerative medicine applications. Part I. Keratinocyte and Organ Cultures Primary Mouse Keratinocyte Culture Annalisa Pirrone, Barbara Hager, and Philip Fleckman Serial Cultivation of Primary Adult Murine Keratinocytes Richard P. Redvers and Pritinder Kaur Keratinocyte Culture in the Absence of Substrate Attachment Monika Jost and Ulrich Rodeck Application of Genetically Modified Feeder Cells for Culture of Keratinocytes Takashi Kameda and Toshihiro Sugiyama Organ Culture of Developing Mouse Skin and Its Application for Molecular Mechanistic Studies of Morphogenesis Mariko Kashiwagi and Nam-ho Huh Experimental Models to Analyze Differentiation Functions of Cultured Keratinocytes In Vitro and In Vivo Nicole Maas-Szabowski, Norbert E. Fusenig, and Hans-Jurgen Stark In Vitro Fabrication of Engineered Human Skin Alexander Margulis, Weitian Zhang, and Jonathan A. Garlick Part II. Epidermal Stem Cells In Vivo Labeling and Analysis of Epidermal Stem Cells Wei-Yang Wu and Rebecca J. Morris Method for the Harvest and Assay of In Vitro Clonogenic Keratinocytes Stem Cells From Mice Wei-Yang Wu and Rebecca J. Morris FACS Enrichment of Human Keratinocyte Stem Cells Amy Li and Pritinder Kaur Isolation, Characterization, and Culture of Epithelial Stem Cells Jackie R. Bickenbach Keratin 19 as a Stem Cell Marker In Vivo and In Vitro Danielle Larouche, Cindy Hayward, Kristine Cuffley, and Lucie Germain Part III. Analysis of Epidermal Differentiation Immunolocalization in the Epidermis Tammy-Claire Troy, Ramtin Rahbar, Bilge Diker, and Kursad Turksen Epidermal Cell Analysis by RT-PCR Tammy-Claire Troy, Robert Man-Kit Cheung, and Kursad Turksen Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis Carolyn Byrne and Matthew J. Hardman Analysis of Early Epidermal Development in Zebrafish Ashley E. Webb and David Kimelman Analysis of E2F Factors During Epidermal Differentiation Wing Y. Chang and Lina Dagnino Analysis of HOX Homeodomain Proteins and Gene Transcripts in the Epidermis Laszlo G. Komuves and Corey Largman Apoptosis in the Epidermis Kiyofumi Yamanishi, Chun-Shen Shen, and Hitoshi Mizutani Fate of Desmosomal Proteins in Apoptotic Epidermal Cells Jorg Weiske and Otmar Huber Analysis of Connexin 43 Expression on Keratinocytes Using Flow Cytometry Maja Matic, Christopher Pullis, Marc G. Golightly and Sanford R. Simon MMP-9 and TIMP-1 Assays in Keratinocyte Cultures Takashi Kobayashi Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins Kenji Kizawa and Mayumi Ito Immunoelectron Microscopic Analysis of Cornified Cell Envelopes and Antigen Retrieval Akemi Ishida-Yamamoto Part IV. Methods and Approaches for the Analysis for Epidermal Function Cell Kinetic Analysis in Artificial Skin Using Immunochemical Methods Andrea Casasco, Antonia Icaro Cornaglia, Federica Riva, Marco Casasco, and Alberto Calligaro Proliferation, Differentiation, and Inflammation in Normal and Hyperproliferative Skin Using Multiparameter Flow Cytometry Piet E. J. van Erp Fluorimetric DNA Assay of Cell Number William R. Otto Keratinocyte Transient Transfections Anthony M. Flores and Brian J. Aneskievich Tetracycline-Regulated Gene Expression in Epidermal Keratinocytes Richard B. Presland and Philip Fleckman Gene Targeting by Oligonucleotides in Keratinocytes Olga Igoucheva and Kyonggeun Yoon Promoter Analysis in the Human SPRR Gene Family David F. Fischer and Claude Backendorf Stable Integration of Large PAC Constructs in Keratinocytes Sarah H. Williams and Alain Hovnanian Targeted Somatic Mutagenesis in the Mouse Epidermis Daniel Metzger, Mei Li, and Pierre Chambon Methods to Study Protein-Protein Interactions Jin-Jun Meng, Meghan Rojas, Willis Bacon, John T. Stickney, and Wallace Ip Isolation of Recombinant Phage-Displayed Antibodies Recognizing Skin Keratinocytes Kim Bak Jensen and Peter Kristensen Analysis of Tissue-Specific DNA Methylation During Development Jun Ohgane, Naka Hattori, and Kunio Shiota Serial Analysis of Gene Expression in Human Keratinocytes and Epidermis Bastiaan J. H. Jansen, Gys de Jongh, Joost Schalkwijk, and Fred van Ruissen Methods for Gene Expression Profiling in Dermatology Research Using DermArray(R) Nylon Filter DNA Microarrays Richard L. Davis, Jr., Rusla M. DuBreuil, Shanker P. Reddy, and Thomas P. Dooley Two-Photon Fluorescence Imaging and Reactive Oxygen Species Detection Within the Epidermis Kerry M. Hanson and Robert M. Clegg Part V. Transplantation and Gene Therapy In Vivo Transplantation of Engineered Human Skin Shari Greenberg, Alexander Margulis, and Jonathan A. Garlick Epidermis-Targeted Gene Transfer Using In Vivo Electroporation Hiroki Maruyama, Jun-Ichi Miyazaki, and Fumitake Gejyo Gene and Stem Cell Therapy of the Hair Follicle Robert M. Hoffman Index