Description
Humana Press Epithelial Cell Culture Protocols 1st Editon 2010 Softbound by Clare Wise
There have been significant advances in research involving the isolation and culture of epithelial cells in the past decade, and many new techniques have been developed. Monolayer cultures can be used to evaluate the nature and behavior of cells, while the use of epithelial cells in model systems has allowed a deeper understanding of cellular and molecular mechanisms and interactions. The aim of this book is to provide a comprehensive, step-by-step guide to many techniques for epithelial cell culture, combining in one volume the more commonly used protocols along with many that are more speci- ized. Epithelial Cell Culture Protocols should help those who are new to this field and want to learn the basic culture techniques, as well as those needing to use more wide ranging and specific protocols. It should be a useful resource on its own, and also complement the other volumes that have been written about cell culture in the Methods in Molecular Biology series. Epithelial Cell Culture Protocols covers a wide variety of protocols, mostly aimed at the researcher, but also a few aimed at clinicians. The est- lishment and maintenance of primary cultures derived from many different tissues and different species is covered. Particular emphasis has been placed on protocols needed to further analyze and assess epithelial cells, for example, by looking at apoptosis and integrins and by measuring membrane capa- tance and confluence. Using different co-culture techniques, it is possible also to develop models to investigate many different systems in vitro. Human Lens Epithelial Cell Culture.- Human Airway Epithelial Cell Culture.- Rat Gastric Mucosal Epithelial Cell Culture.- Thymic Epithelial Cell Culture.- Bile Duct Epithelial Cell Culture.- Liver Epithelial Cell Culture.- Pulmonary Epithelial Cell Culture.- Prostate Epithelial Cell Isolation and Culture.- A Bovine Mammary Endothelial/Epithelial Cell Culture Model of the Blood/Milk Barrier.- The Blood-CSF Barrier in Culture.- An In Vitro Model of Differentiated Human Airway Epithelia.- Primary Mouse Keratinocyte Culture.- Analyzing Apoptosis in Cultured Epithelial Cells.- Keratins as Markers of Epithelial Cells.- Epithelial Cell Integrins.- Isolation, Cultivation, and Differentiation of Normal Human Epidermal Keratinocytes in Serum-Free Medium.- Clinical Application of Autologous Cultured Epithelia for the Treatment of Burns and Disfigurement of Skin Surfaces.- Transplantation of Cultured Epithelial Cells.- An Outgrowth Culture System of Normal Human Epithelium.- Application of Epithelial Cell Culture in Drug Transport in the Respiratory Tract.- Applications of Epithelial Cell Culture in Studies of Drug Transport.- X-Ray Microanalysis of Epithelial Cells in Culture.- ENU Mutagenesis of Rat Mammary Epithelial Cells.- ENU-Induced Ovarian Cancer.- Measurement of Membrane Capacitance in Epithelial Monolayers.- Assessing Epithelial Cell Confluence by Spectroscopy.- Co-Cultivation of Liver Epithelial Cells with Hepatocytes.- Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium.- Establishing Epithelial-Immune Cell Co-Cultures.- Explant Cultures of Embryonic Epithelium.- Bacterial Interactions with Host Epithelium In Vitro.