Description
Humana Epithelial Cell Culture Protocols by Clare Wise
Well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. The emphasis is on the analysis and assessment of epithelial cells, for example by looking at apoptosis and integrins, or by measuring membrane capacitance and confluence. Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. Epithelial Cell Culture Protocols offers a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells. Human Lens Epithelial Cell Culture Nobuhiro Ibaraki Human Airway Epithelial Cell Culture Mutsuo Yamaya, Masayoshi Hosoda, Tomoko Suzuki, Norihiro Yamada, and Hidetada Sasaki Rat Gastric Mucosal Epithelial Cell Culture Yoshitaka Konda and Tsutomu Chiba Thymic Epithelial Cell Culture Carsten Ropke Bile Duct Epithelial Cell Culture Alphonse E. Sirica Liver Epithelial Cell Culture Rolf Gebhardt Pulmonary Epithelial Cell Culture Ben Forbes Prostate Epithelial Cell Isolation and Culture David L. Hudson A Bovine Mammary Endothelial/Epithelial Cell Culture Model of the Blood/Milk Barrier Albert Guidry and Celia O'Brien The Blood-CSF Barrier in Culture: Development of a Primary Culture and Transepithelial Transport Model for Choroidal Epithelial Cells Wei Zheng and Qiuqu Zhao An In Vitro Model of Differentiated Human Airway Epithelia: Methods for Establishing Primary Cultures Philip H. Karp, Thomas O. Moninger, S. Pary Weber, Tamara S. Nesselhauf, Janice L. Launspach, Joseph Zabner, and Michael J. Welsh Primary Mouse Keratinocyte Culture Kairbaan Hodivala-Dilke Analyzing Apoptosis in Cultured Epithelial Cells Andrew P. Gilmore and Charles H. Streuli Keratins as Markers of Epithelial Cells David L. Hudson Epithelial Cell Integrins Joachim Rychly Isolation, Cultivation, and Differentiation of Normal Human Epidermal Keratinocytes in Serum-Free Medium Sebastian Zellmer and Dieter Reissig Clinical Application of Autologous Cultured Epithelia for the Treatment of Burns and Disfigurement of Skin Surfaces Norio Kumagai Transplantation of Cultured Epithelial Cells Minoru Ueda, Yukio Sumi, Yoshitaka Hibino, and Ken-Ichiro Hata An Outgrowth Culture System of Normal Human Epithelium: An In Vitro Model to Study the Coordination of Cellular Migration and Proliferation Tohru Masui Application of Epithelial Cell Culture in Drug Transport in the Respiratory Tract Jie Shen and Vincent H. L. Lee Applications of Epithelial Cell Culture in Studies of Drug Transport Staffan Tavelin, Johan Grasjo, Jan Taipalensuu, Goran Ocklind, and Per Artursson X-Ray Microanalysis of Epithelial Cells in Culture Godfried M. Roomans ENU Mutagenesis of Rat Mammary Epithelial Cells George Stoica ENU-Induced Ovarian Cancer George Stoica Measurement of Membrane Capacitance in Epithelial Monolayers Carol A. Bertrand and Ulrich Hopfer Assessing Epithelial Cell Confluence by Spectroscopy Simon A. Lewis Co-Cultivation of Liver Epithelial Cells with Hepatocytes Rolf Gebhardt Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium Wee Soo Shin, Chieko Hemmi, and Teruhiko Toyo-oka Establishing Epithelial-Immune Cell Co-Cultures: Effects on Epithelial Ion Transport and Permeability Derek M. McKay and Mary H. Perdue Explant Cultures of Embryonic Epithelium: Analysis of Mesenchymal Signals Carin Sahlberg, Tuija Mustonen, and Irma Thesleff Bacterial Interactions with Host Epithelium In Vitro Nicola Jones, Mary H. Perdue, Philip M. Sherman, and Derek M. McKay