Description
SPRINGER Essential Genes And Genomes Methods And Protocols (Hb 2022) by ZHANG R.
This book provides state-of-the-art information on gene essentiality screenings in a wide variety of organisms, i.e. screening for protein-coding genes and other genomic elements that are required by an organism to survive under specific conditions. With a focus on the two techniques that have revolutionized the field, the collection begins with chapters employing CRISPR/Cas9-based approaches followed by Tn-seq-based approaches, but later chapters also delve into other techniques for exploring essential genes, such as bioinformatics methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Essential Genes and Genomes: Methods and Protocols is an ideal guide for researchers attempting to strip genetics down to its fundamentals. 1. A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9Barbara Mair, Michael Aregger, Amy H.Y. Tong, Katherine S.K. Chan, and Jason Moffat2. CRISPR/Cas9 Screening to Identify Conditionally Essential Genes in Human Cell LinesKimberly S. Huggler, Nicholas J. Rossiter, Kyle M. Flickinger, and Jason R. Cantor3. Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential GenesBert Kwanten, Jasper Neggers, and Dirk Daelemans4. Design and Generation of a CRISPR Interference System for Genetic Repression and Essential Gene Analysis in the Fungal Pathogen Candida albicansLauren Wensing and Rebecca S. Shapiro5. Identification of Essential Genes Using Sequential CRISPR and siRNA ScreensLuke DeHart, Oliver P. Yockey, and Jesse Bakke6. A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell LinesKohei Nishimura and Tatsuo Fukagawa7. New Method for Genome-Scale Functional Genomics Study in Bacteria with Superior Performance: CRISPR Interference ScreenXihao Liao, Xin-Hui Xing, and Chong Zhang8. A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in CyanobacteriaJu-Yuan Zhang, Tian-Cai Niu, Gui-Ming Lin, and Cheng-Cai Zhang9. A Method of Transposon Insertion Sequencing in Comprehensively Identifying Vibrio vulnificus Genes Required for Growth in Human SerumMiguel Carda-Dieguez and Carmen Amaro10. The Use of Tn-Seq and the FiTnEss Analysis to Define the Core Essential Genome of Pseudomonas aeruginosaBradley E. Poulsen, Anne E. Clatworthy, and Deborah T. Hung11. Analysis of Escherichia coli K1 Virulence Genes by Transposon-Directed SequencingAlex J. McCarthy and Peter W. Taylor12. Using Genome Scale Mutant Libraries to Identify Essential GenesKevin S. Myers, Piyush Lal, Daniel R. Noguera, and Timothy J. Donohue13. Identification and Analysis of Essential Genes in Streptococcus mutans with Transposon SequencingAlejandro R. Walker and Robert C. Shields14. The Use of TnSeq to Identify Essential Alphaproteobacterial Genes Reveals Operational Variability in Conserved Developmental and Cell Cycle SystemsGayatri Sharma and Patrick D. Curtis15. A Proposed Framework to Identify Dispensable and Essential Functions in Bifidobacteria: Case Study of Bifidobacterium breve UCC2003 as a Prototype of Its GenusLorena Ruiz, Francesca Bottacini, Lucie Semenec, Amy Cain, and Douwe van Sinderen16. The Application of Transposon Insertion Sequencing in Identifying Essential Genes in B. fragilis Yaligara Veeranagouda and Hannah M. Wexler17. Repressible Promoter System to Study Essential Genes in MycobacteriaFrancesca Boldrin and Riccardo Manganelli18. A Single-Cell RNA-Seq-Based Approach for Genome-Wide Identification of Cell Essential GenesYuqiu Lu and Shaolin Shi19. Identification of Essential Genes in Caenorhabditis elegans with Lethal Mutations Maintained by Genetic BalancersShicheng Yu, Chaoran Zheng, and Jeffrey Shih-Chieh Chu20. A Screening Method to Identify Essential Yeast Genes for Responses Towards SpermineMustapha Aouida and Dindial Ramotar21. An Effective Pre-Processing Method for High-Quality Pan-Genome Analysis of Bacillus subtilis and Escherichia coliHao Wu, Zhi-Kai Yang, Tong Yang, Dan Wang, Hao Luo, and Feng Gao22. Analysis of Gene Essentiality from TnSeq Data Using TransitThomas R. Ioerger23. Geptop 2.0: Accurately Select Essential Genes from the List of Protein-Coding Genes in Prokaryotic GenomesQing-Feng Wen, Wen Wei, and Feng-Biao Guo