Description
Oxford Experimental Protocols for Reactive Oxygen and Nitrogen Species 2000 Edition by Naoyuki Taniguchi, John Gutteridge
Experimental Protocols for Reactive Oxygen and Nitrogen Species is a translated, expanded, and fully updated of the Japanese book Experimental Protocols for Reactive Oxygen Research: assay methods, gene analysis, and pathophysiology models published in 1994. The aim of the book is to provide experimental protocols covering many aspects of free radical research: biochemistry, molecular and cellular biology, genetics, physiology, and medicine. Theprotocols are all self-contained describing the equipment and reagents needed and then detailing the experimental procedure. Table of contents :- PART ONE: MEASUREMENT ; ROS and RNS measurements ; Measurements for proteinaceous plasma antioxidants, low Mw antioxidants, and REDOX related compounds ; Measurements for specific ROS and RNS enzymes and related proteins ; Lipids and lipid peroxidization ; Iron and reactive iron species ; Copper and reactive copper species ; PART TWO: GENE ANALYSES ; Research for gene expressions of ROS and RNS related genes ; DNA damage and repair ; PART THREE: EXPERIMENTS FOR PATHOPHYSIOLOGICAL MODELS ; Isolated and perfused hearts (Langendorf's model) ; Myocardial ischaemia-reperfusion models in vivo ; GI tracts in vivo ; Brain ischaemia ; Cell culture ; Athersclerosis ; Dicarbonyls and HB-EGF gene expression UV damage ; Ageing ; Ovulation ; Scavenging NO in experimental animals ; Using radioimmuno assays in brain slices ; Oxidative stress ; Carcinogenesis ; Iron overload ; Copper overload