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Food-Borne Pathogens Methods And Protocols by Catherine Adley , Springer

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  • General Information  
    Author(s)Catherine Adley
    Publish YearNovember 2005


    Springer Food-Borne Pathogens Methods And Protocols by Catherine Adley

    A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls._x000D_ _x000D_Part I. The Bacteria_x000D_ _x000D_ Detection of Hemolysins in Aeromonas spp. Isolates From Food Sources: PCR Analysis and Biological Activity_x000D_ Rosabel Falcon, Tatiana dAlbuquerque e Castro, Maria das Gracas de Luna, Angela Correa de Freitas-Almeida, and Tomomasa Yano_x000D_ _x000D_ Detection and Purification of Bacillus cereus Enterotoxins_x000D_ Toril Lindback and Per Einar Granum_x000D_ _x000D_ Campylobacter: Isolation, Identification, and Preservation_x000D_ Rachel Gorman and Catherine C. Adley_x000D_ _x000D_ Detection of Clostridium botulinum by Multiplex PCR in Foods and Feces_x000D_ Miia Lindstroem, Mari Nevas, and Hannu Korkeala_x000D_ _x000D_ Multiplex PCR for Specific Identification of Enterohemorrhagic Escherichia coli Strains in the O157:H7 Complex_x000D_ Peter C. H. Feng and Steven R. Monday_x000D_ _x000D_ PulseNets Step-by-Step Laboratory Protocol for Molecular Subtyping of Listeria monocytogenes by Macrorestriction and Pulsed-Field Gel Electrophoresis_x000D_ Lewis M. Graves and Balasubramanian Swaminathan_x000D_ _x000D_ Plesiomonas shigelloides: Detection by PCR_x000D_ Ivan Ciznar, Carlos Gonzalez-Rey, Karel Krovacek, and Anna Hostacka_x000D_ _x000D_ Pulsed-Field Gel Electrophoresis As a Molecular Technique in Salmonella Epidemiological Studies_x000D_ Rachel Gorman and Catherine C. Adley_x000D_ _x000D_ Kits for Detection of Food Poisoning Toxins Produced by Bacillus cereus and Staphylococcus aureus_x000D_ Moira M. Brett_x000D_ _x000D_ Microbiological and Molecular Methods to Identify and Characterize Toxigenic Vibrio cholerae From Food Samples_x000D_ Keya De, Ranjan K. Nandy, and G. Balakrish Nair_x000D_ _x000D_ HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes_x000D_ Jennifer Colford_x000D_ _x000D_ Part II. The Viruses_x000D_ _x000D_ Detection of Noroviruses of Genogroups I and II in Drinking Water by Real-Time One-Step RT-PCR_x000D_ Christian M. Beuret_x000D_ _x000D_ Detection of Enteroviruses_x000D_ Miguel-Angel Jimenez-Clavero, Victoria Ley, Nuria Gomez, and Juan-CarlosSaiz_x000D_ _x000D_ Detection of Hepatitis A Virus and Rotavirus Using Nucleic Acid Sequence-Based Amplification_x000D_ Julie Jean and Ismail Fliss_x000D_ _x000D_ Part III. The Parasites_x000D_ _x000D_ Isolation and Characterization of Cathepsin-L1 Protease From Fasciola hepatica Excretory-Secretory Products for Serodiagnosis of Human Fasciolosis_x000D_ Sandra M. ONeill, Grace Mulcahy, and John P. Dalton_x000D_ _x000D_ Molecular Biology Methods for Detection and Identification of Cryptosporidium Species in Feces, Water, and Shellfish_x000D_ Colm J. Lowery, L. Xiao, U. M. Ryan, James S. G. Dooley, B. Cherie Millar, and John E. Moore_x000D_ _x000D_ Molecular Identification of Nematode Worms From Seafood (Anisakis spp. and Pseudoterranova spp.) and Meat (Trichinella spp.)_x000D_ Giuseppe La Rosa, Stefano DAmelio, and Edoardo Pozio_x000D_ _x000D_ Part IV. Parallel Studies to the Analysis of Food-Borne Pathogens_x000D_ _x000D_ Approaches to Developing Quantitative Risk Assessment Models_x000D_ Enda J. Cummins_x000D_ _x000D_ A Review of Surveillance Networks of Food-Borne Diseases_x000D_ Camelia Molnar, Rita Wels, and Catherine C. Adley_x000D_ _x000D_ Index_x000D_