Description
Humana Hepatocellular Carcinoma Methods And Protocols by Nagy A. Habib
Hepatocellular Carcinoma: Methods and Protocols outlines the research methods applied in the laboratories and clinics of those scientists and cli- cians interested in the understanding and clinical management of patients with hepatocellular carcinoma (HCC). Part I, The Clinical Problem, has been contributed by two leading cli- cal groups who have identified and addressed problem areas related to the management of HCC patients. Various treatment modalities are discussed and emphasis is placed on the limitations they experienced. Part II, HCC Carcinogenesis, reviews the main etiological factors related to hepatitis B and hepatitis C. Part III, Molecular and Biological Characteristics, provides insight into the molecular changes associated with HCC, including tumor-suppressor genes, oncogenes, adhesion molecules, matrix metalloproteinase, and novel genes and markers. Part IV, HCC Gene Therapy, addresses gene therapy approaches to treating hepatocellular carcinoma. It includes the use of various vectors, such as lipids, viruses such as adenoviruses and baculoviruses, and virus detection using el- tron microscopy assessment.The use of adenovirus with specific promotors, such as AFP, is also included. Preclinical and clinical data on the killing of cancer cells using tumor-suppressor genes, antisense to growth factors, immunogene therapy, or virus-directed enzyme prodrug therapy are addressed. Part I. The Clinical Problem. Hepatocellular Carcinoma: The Clinical Problem, Valery Usatoff and Nagy A. Habib. Medical Management of Hepatocellular Carcinoma, Stephen M. Riordan and Roger Williams. Part II. HCC Carcinogenesis. Possible Involvement of the NS3 Protein of Hepatitis C Virus in Hepatocarcinogenesis: Its Interaction with the p53 Tumor Suppressor, Satoshi Ishido, Tsunenori Fujita, and Hak Hotta. Abrogation of p53-Induced Apoptosis by the Hepatitis B Virus X Gene, Xin Wei Wang. Hepatocellular Carcinoma: Role of Hepatitis Viruses and Liver Cell Dysplasia, Carmen Vandelli and Francesco Renzo. Part III. Molecular and Biological Characteristics. Impact of Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV): From Research to Clinical Practice, Maurizia Rossana Brunetto, Filippo Oliveri, and Ferruccio Bonino. Mutation of p53 Tumor Suppressor Gene in Hepatocellular Carcinoma, Apollonia Tullo and Elisabetta Sbisa. Diagnostic Markers in Hepatocellular Carcinoma Using Immunohistochemical Techniques, Yaw Ohene-Abuakwa and Massimo Pignatelli. Assessing Matrix Metalloproteinase Expression and Activity in Hepatocellular Carcinomas, Orlando Musso, Bruno Clement, and Nathalie Theret. Isolation of Novel Markers for Hepatocellular Carcinoma by a Subtraction-Enhanced Display Technique, Chuan-Ging Wu. Measurement of Protein Expression of p53, p21WAF1, and Rb in Patients with Surgically Treated Hepatocellular Carcinoma by Using Catalyzed Signal-Amplication System, Takuji Naka, Tetsuya Kaneko, and Nobuaki Kaibara. Part IV. HCC Gene Therapy. Gene Therapy Vectors Harboring AFP Regulatory Sequences: Preparation of an Adenoviral Vector, Shuichi Kaneko and Taiki Tamaoki. Advantages and Disadvantages of Multiple Different Methods of Adenoviral Vector Construction, Prem Seth and James Higginbotham. Electron Microscopic Assessment of Adenovirus-Mediated Transfer, Catherine E. Sarraf. p53 Plasmid Preparation and Techniques for Analysis of Gene Transfer and Expression, Ragai R. Mitry,Michael D. Kelly, Jian Zhao, Satoko Negishi, Marc R. Mansour, and Nagy A. Habib. Antisense IGF-I for Hepatocellular Carcinoma, Yanjun Liu, Jian Zhao, Yang Lu, Jerzy Trojan, Mengchao Wu, and Yajun Guo. Novel Effective Tumor Vaccines for Hepatocellular Carcinoma, Lixin Wei, Hao Wang, Yanjun Liu, Mengchao Wu, and Yajun Guo. Immunoglobulin Fusion Proteins as a Tool for Evaluation of T-Cell Costimulatory Molecules, Andrei I. Chapoval, Gefeng Zhu, and Lieping Chen. Adenovirus-Mediated Drug Sensitivity Gene Therapy for Hepatocelular Carcinoma, Fumihiko Kanai, Makoto Ohashi, Paola A. Marignani, Yasushi Shiratori, Hirofumi Hamada, and Masao Omata. Part V. Clinical Protocol for p53 Gene Therapy for Liver Tumors. Clinical Protocol for p53 Gene Therapy for Liver Tumors, Michael D. Kelly, Ragai R. Mitry, and Nagy A. Habib. Index.