Human Embryogenesis Methods And Protocols (Methods In Molecular Biology Volume 550) by LAFOND JULIE ET.AL, HUMANA PRESS

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HUMANA PRESS Human Embryogenesis Methods And Protocols (Methods In Molecular Biology Volume 550) by LAFOND JULIE ET.AL

Despite its inherent controversy, the exploration of the human embryo can unlock many of the answers to our deepest biological questions. In Human Embryogenesis: Methods and Protocols, internationally recognized researchers contribute detailed methods to analyze various aspects of the embryogenesis process. While comprehensively covering subjects such as the molecular mechanisms of embryonic development, in vitro fertilization, cloning, and the laws and ethical considerations of working with embryos, the volume also addresses critical features of fetal and placental development as well as of uterine biology. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls._x000D__x000D__x000D_Authoritative and state-of-the-art, Human Embryogenesis: Methods and Protocols provides a firm foundation for the successful analysis of the embryogenesis process and an easily accessible description of the limitations and advantages of the techniques proposed, certain to aid all those who wish to further unravel the mysteries of human embryogenesis._x000D_ _x000D_ Part I. Introduction: What is Human Embryogenesis?_x000D_ _x000D_ _x000D_ Human Embryogenesis: Overview _x000D_ _x000D_ Cathy Vaillancourt and Julie Lafond_x000D_ _x000D_ Part II. Studying Embryo Implantation: from Trophoblasts to Embryo_x000D_ _x000D_ Differential Gene Expression in the Uterus and Blastocyst During the Reactivation of Embryo Development in a Model of Delayed Implantation. _x000D_ _x000D_ Pavine L.C. Lefevre and Bruce D. Murphy_x000D_ _x000D_ Coculture of Decidua and Trophoblast to Study Proliferation and Invasion _x000D_ _x000D_ Marie Cohen and Paul Bischof _x000D_ _x000D_ Isolation and Culture of Term Human Cytotrophoblast Cells and In Vitro Methods for Studying Human Cytotrophoblast Cells Calcium Uptake _x000D_ _x000D_ Frederique Le Bellego, Cathy Vaillancourt and Julie Lafond_x000D_ _x000D_ Endocrinology and Cell Signaling in Human Villous Trophoblast _x000D_ _x000D_ Catherine Mounier, Benoit Barbeau, Cathy Vaillancourt and Julie Lafond_x000D_ _x000D_ Gestation-Induced Uterine Vascular Remodeling _x000D_ _x000D_ Pierre-Andre Scott, Mylene Provencher, Pascale Guerin and Jean St-Louis _x000D_ _x000D_ Part III. Steriodogenesis and Reproductive System_x000D_ _x000D_ Placental and Fetal Steriodogenesis _x000D_ _x000D_ J. Thomas Sanderson_x000D_ _x000D_ Current Methods in Investigating the Development of the Female Reproductive System _x000D_ _x000D_ Patrick J. Devine, Patricia B. Hoyer and Aileen F. Keating_x000D_ _x000D_ A Revised and Improved Method for the Isolation of Seminiferous Tubule-Enriched Fractions that Preserves the Phosphorylated and Glycosylated Forms of Proteins _x000D_ _x000D_ Casimir D. Akpov and R.-Marc Pelletier_x000D_ _x000D_ A Novel Technical Approach for the Measurement of Individual ACAT-1 and ACAT-2 Enzymatic Activity in the Testis _x000D_ _x000D_ Li Chen, Julie Lafond and R.-Marc Pelletier_x000D_ _x000D_ Part IV. From Embryo to Fetal Development _x000D_ _x000D_ GeneticDissection of Caenorhabditis elegans Embryogenesis Using RNA Interference and Flow-Cytometry_x000D_ _x000D_ Elodie L. Boulier and Sarah Jenna_x000D_ _x000D_ Immune System: Maturation of Myeloid Cells _x000D_ _x000D_ Jamila Ennaciri and Denis Girard _x000D_ _x000D_ Functional Development of Human Fetal Gastrointestinal Tract_x000D_ _x000D_ Emile Levy, Edgard Delvin, Daniel Menard and Jean-Francois Beaulieu_x000D_ _x000D_ Renal and Cardiac Na+-K+-ATPase and Aconitase in a Rat Model of Fetal Programming_x000D_ _x000D_ Rebecca Gaudet and Michele Brochu _x000D_ _x000D_ Part V. Embryotoxicity, In Vitro Fertilization, Cloning, and Ethics_x000D_ _x000D_ Assessment of Embryotoxicity Using Mouse Embryo Culture_x000D_ _x000D_ Louise M. Winn and Emily W.Y. Tung_x000D_ _x000D_ Fertilization In Vitro _x000D_ _x000D_ Yinzhong Bing and Rodney J. Ouellette_x000D_ _x000D_ Animal Cloning by Somatic Cell Nuclear Transfer _x000D_ _x000D_ Lawrence C. Smith and Jae-Gyu Yoo_x000D_ _x000D_ The Human Embryo: Ethical and Legal Aspects _x000D_ _x000D_ Bartha Maria Knoppers, Sylvie Bordet and Rosario Isasi_x000D_