Human Papillomaviruses Methods And Protocols by Clare Davy, John Doorbar , Springer

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Springer Human Papillomaviruses Methods And Protocols by Clare Davy, John Doorbar

Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular Medicine (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls._x000D_ Identification of New Papillomavirus Types_x000D_ Ethel-Michele de Villiers, Corinna Whitley, and Karin Gunst_x000D_ _x000D_ Identification of HPV Variants_x000D_ John Cason, Jon Bible, and Christine Mant_x000D_ _x000D_ Histochemical Analysis of Cutaneous HPV-Associated Lesions_x000D_ Kiyofumi Egawa_x000D_ _x000D_ Histological Analysis of Cervical Intraepithelial Neoplasia_x000D_ Michael Babawale, Rashmi Seth, Adam Christian, Wessam Al-Utayem, Ragini Narula, and David Jenkins_x000D_ _x000D_ Detection of Papillomavirus Proteins and DNA in Paraffin-Embedded Tissue Sections_x000D_ Woei Ling Peh and John Doorbar_x000D_ _x000D_ Detection and Quantitation of HPV Gene Expression Using Real-Time PCR_x000D_ Rashmi Seth, John Rippin, Li Guo, and David Jenkins_x000D_ _x000D_ Analysis of p16INK4a and Integrated HPV Genomes as Progression Markers_x000D_ Svetlana Vinokurova, Nicolas Wentzensen, and Magnus von Knebel Doeberitz_x000D_ _x000D_ Use of Biomarkers in the Evaluation of CIN Grade and Progression of Early CIN_x000D_ Jan P. A. Baak and Arnold-Jan Kruse_x000D_ _x000D_ HPV DNA Detection and Typing in Cervical Scrapes_x000D_ Peter J. F. Snijders, Adriaan J. C. van den Brule, Marcel V. Jacobs, Rene P. Pol, and Chris J. L. M. Meijer_x000D_ _x000D_ HPV DNA Detection and Typing in Inapparent Cutaneous Infections and Premalignant Lesions_x000D_ Maurits de Koning, Linda Struijk, Mariet Feltkamp, and Jan ter Schegget_x000D_ _x000D_ Establishing HPV-Containing Keratinocyte Cell Lines From Tissue Biopsies_x000D_ Margaret Anne Stanley_x000D_ _x000D_ Using an Immortalized Cell Line to Study the HPV Life Cycle in Organotypic Raft Cultures_x000D_ Paul F. Lambert, Michelle A. Ozbun, Asha Collins, Sigrid Holmgren, Denis Lee, and Tomomi Nakahara_x000D_ _x000D_ Differentiation of HPV-Containing Cells Using Organotypic Raft Culture or Methylcellulose_x000D_ Regina Wilson and Laimonis A. Laimins_x000D_ _x000D_ Propagation of Infectious, High-Risk HPV in Organotypic Raft Culture_x000D_ Margaret E. McLaughlin-Drubin and Craig Meyers_x000D_ _x000D_ Retrovirus-Mediated GeneTransfer to Analyze HPV Gene Regulation and Protein Functions in Organotypic Raft Cultures_x000D_ N. Sanjib Banerjee, Louise T. Chow, and Thomas R. Broker_x000D_ _x000D_ The HPV Xenograft Severe Combined Immunodeficiency Mouse Model_x000D_ William Bonnez_x000D_ _x000D_ The Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced Disease_x000D_ Janet L. Brandsma_x000D_ _x000D_ Studying the HPV Life Cycle in 3A Trophoblasts and Resulting Pathophysiology_x000D_ Yong Liu, Hong You, and Paul L. Hermonat_x000D_ _x000D_ Replication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiae_x000D_ Peter C. Angeletti_x000D_ _x000D_ Analysis of the Regulation of Viral Transcription_x000D_ Bernd Gloss, Mina Kalantari, and Hans-Ulrich Bernard_x000D_ _x000D_ Analysis of HPV Transcription by RPA_x000D_ Jason M. Bodily and Craig Meyers_x000D_ _x000D_ Analysis of Regulatory Motifs Within HPV Transcripts_x000D_ Sarah A. Cumming and Sheila V. Graham_x000D_ _x000D_ Detection of HPV Transcripts by Nested RT-PCR_x000D_ Christine Mant, Barbara Kell, and John Cason_x000D_ _x000D_ Analysis of HPV DNA Replication Using Transient Transfection and Cell-Free Assays_x000D_ Biing Yuan Lin, Thomas R. Broker, and Louise T. Chow_x000D_ _x000D_ Detection and Quantitation of HPV DNA Replication by Southern Blotting and Real-Time PCR_x000D_ Iain M. Morgan and Ewan R. Taylor_x000D_ _x000D_ Analysis of E7/Rb Associations_x000D_ Sandra Caldeira, Wen Dong, and Massimo Tommasino_x000D_ _x000D_ Transformation Assays for HPV Oncoproteins_x000D_ Paola Massimi and Lawrence Banks_x000D_ _x000D_ Analysis of Adeno-Associated Virus and HPV Interaction_x000D_ Paul L. Hermonat, Hong You, C. Maurizio Chiriva-Internati, and Yong Liu_x000D_ _x000D_ In Vitro Assays of Substrate Degradation Induced by High-Risk HPV E6 Oncoproteins_x000D_ Miranda Thomas and Lawrence Banks_x000D_ _x000D_ Measuring the Induction or Inhibition of Apoptosis by HPV Proteins_x000D_ Anna M. Kowalczyk, Geraldine E. Roeder, Katie Green, David J. Stephens, and Kevin Gaston_x000D_ _x000D_ Codon Optimization of Papillomavirus G_x000D_