Immobilization Of Enzymes And Cells by Jose M. Guisan , Springer

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Springer Immobilization Of Enzymes And Cells by Jose M. Guisan

Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation-from the laboratory to industry-of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related propertie _x000D_Immobilization of Enzymes as the 21st Century Begins: An Already Solved Problem or Still an Exciting Challenge?_x000D_ Jose M. Guisan_x000D_ _x000D_ Immobilization of Enzymes: A Literature Survey_x000D_ Beatriz M. Brena and Franciso Batista-Viera_x000D_ _x000D_ Cross-Linked Enzyme Aggregates_x000D_ Roger A. Sheldon, Rob Schoevaart, and Luuk M. van Langen_x000D_ _x000D_ Immobilization-Stabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups_x000D_ Cesar Mateo, Olga Abian, Gloria Fernandez-Lorente, Benevides C. C. Pessela, Valeria Grazu, Jose M. Guisan, and Roberto Fernandez-Lafuente_x000D_ _x000D_ Glutaraldehyde in Protein Immobilization: A Versatile Reagent_x000D_ Lorena Betancor, Fernando Lopez-Gallego, Noelia Alonso-Morales, Gisella Dellamora, Cesar Mateo, Roberto Fernandez-Lafuente, and Jose M. Guisan_x000D_ _x000D_ Practical Protocols for Lipase Immobilization Via Sol-Gel Techniques_x000D_ Manfred T. Reetz_x000D_ _x000D_ Encapsulation of Enzymes Using Polymers and Sol-Gel Techniques_x000D_ Monica Campas and Jean-Louis Marty_x000D_ _x000D_ Design of Smart Biocatalysts: Immobilization of Enzymes on Smart Polymers_x000D_ Ipsita Roy and Munishwar N. Gupta_x000D_ _x000D_ Affinity Immobilization of Tagged Enzymes_x000D_ Silvana Andreescu, Bogdan Bucur, and Jean-Louis Marty_x000D_ _x000D_ Bioaffinity Immobilization_x000D_ Ipsita Roy and Munishwar N. Gupta_x000D_ _x000D_ One-Step Purification, Immobilization, and Stabilization of Poly-Histidine-Tagged Enzymes Using Metal Chelate-Epoxy Supports_x000D_ Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Rodrigo Torres, Fernando Lopez-Gallego, Jose M. Guisan, and Roberto Fernandez-Lafuente_x000D_ _x000D_ Stabilization of Multimeric Enzymes Via Immobilization and Further Cross-Linking With Aldehyde-Dextran_x000D_ Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo, Gloria Fernandez-Lorente, Roberto Fernandez-Lafuente, and Jose M. Guisan_x000D_ _x000D_ Purification, Immobilization,Hyperactivation, and Stabilization of Lipases by Selective Adsorption on Hydrophobic Supports_x000D_ Jose M. Palomo, Gloria Fernandez-Lorente, Cesar Mateo, Rosa L. Segura, Claudia Ortiz, Roberto Fernandez-Lafuente, and Jose M. Guisan_x000D_ _x000D_ Immobilization and Stabilization of Proteins by Multipoint Covalent Attachment on Novel Amino-Epoxy-Sepabeads (R)_x000D_ Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Fernando Lopez-Gallego, Rodrigo Torres, Manuel Fuentes, Aurelio Hidalgo, Jose M. Palomo, Lorena Betancor, Gloria Fernandez-Lorente, Claudia Ortiz, Olga Abian, Jose M. Guisan, and Roberto Fernandez-Lafuente_x000D_ _x000D_ Improved Stabilization of Chemically Aminated Enzymes Via Multipoint Covalent Attachment on Glyoxyl Supports_x000D_ Tamara Montes, Fernando Lopez-Gallego, Manuel Fuentes, Cesar Mateo, Valeria Grazu, Lorena Betancor, Jose M. Guisan, and Roberto Fernandez-Lafuente_x000D_ _x000D_ Stabilization of New Imprint Property of Glucose Oxidase in Pure Aqueous Medium by Cross-Linked-Imprinting Approach_x000D_ Alankar Vaidya and Lutz Fischer_x000D_ _x000D_ Reversible Covalent Immobilization of Enzymes Via Their Thiol Groups_x000D_ Francisco Batista-Viera, Karen Ovsejevi, and Carmen Manta_x000D_ _x000D_ Very Strong But Reversible Immobilization of Enzymes on Supports Coated With Ionic Polymers_x000D_ Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Claudia Ortiz, Fernando Lopez-Gallego, Lorena Betancor, Noelia Alonso-Morales, Jose M. Guisan, and Roberto Fernandez-Lafuente_x000D_ _x000D_ Immobilization of Enzymes on Magnetic Particles_x000D_ Martina Koneracka, Peter Kopcansky , Milan Timko, Chenyl Nynitapal Ramchand, Zainul M. Saiyed, Michael Trevan, and Anil de Sequeira_x000D_ _x000D_ Immobilization of Proteins on Gold Surfaces_x000D_ Jose M. Abad, Marcos Pita, and Victor M. Fernandez_x000D_ _x000D_ Immobilization of Enzymes on Electrodes_x000D_ Gilvanda Silva Nunes and Jean-Louis Marty_x000D_ _x000D_ Immobilization of Enzymes for Use in Organic Media_x000D_ Patrick_x000D_