Description
Humana Press Lymphoma by Tim Illidge , Peter W. M. Johnson
A cutting-edge collection of readily reproducible molecular techniques to better understand, classify, and treat lymphoma. Among the highlights are methods to use immunoglobulin gene rearrangements as markers of clonality, to exploit patterns of somatic mutation in the variable regions to indicate at which stage transformation occurred, and to apply gene arrays to the question of biological heterogeneity in morphologically similar diseases. Research methodologies that are highly likely to become routine practice in the future, such as DNA microarray and immunoglobulin V-gene rearrangements, and measurement of minimal disease, are included. There are also molecular techniques for providing for producing novel therapeutics, such as a DNA vaccine with patient-specific sequences derived from the lymphoma in question.show more Purification of Primary Malignant B-Cells and Immunoblot Analysis of Bcl-2 Family ProteinsClaire Dallman and Graham PackhamMolecular Diagnosis of Lymphoma: Outcome Prediction by Gene Expression Profiling in Diffuse Large B-Cell LymphomaKim Last, Silvana Debarnardi, and T. Andrew ListerDemonstration of a Germinal Center Immunophenotype in Lymphomas by Immunocytochemistry and Flow CytometryAndrew Jack, Sharon Barrans, David Blythe, and Andrew RawstronKaryotyping Lymph Node Biopsies in Non-Hodgkin's LymphomaFiona M. Ross and Christine J. HarrisonIdentification of Lymphoma-Associated Antigens Using SEREXAmanda P. Liggins, Barbara A. Guinn, and Alison H. BanhamDetermining Mutational Status of Immunoglobulin V Genes in Chronic Lymphocytic Leukemia: A Useful Prognostic IndicatorSurinder S. Sahota, Gavin Babbage, Niklas Zojer, Christian H. Ottensmeier, and Freda K. StevensonIdiotype Gene Rescue in Follicular LymphomaKaty McCann, Surinder S. Sahota, Freda K. Stevenson, and Christian H. OttensmeierImmunoglobulin Gene Mutation Patterns and Heterogeneity of Marginal Zone LymphomaMariella Dono and Manlio FerrariniT-Cell Receptor Molecular Diagnosis of T-Cell LymphomaElizabeth Hodges, Anthony P. Williams, Susan Harris, and John L. SmithCloning of Immunoglobulin Chromosomal Translocations by Long-Distance Inverse Polymerase Chain ReactionE. Loraine Karran, Takashi Sonoki, and Martin J. S. DyerSequential Fluorescence In Situ Hybridization Analysis for Trisomy 12 in B-Cell Chronic Lymphocytic LeukemiaViktoria HjalmarSplenic Marginal Zone Lymphoma: 7q AbnormalitiesRachel E. Ibbotson, Anton E. Parker, and David G. OscierBCL-6: Rearrangement and Mutation in LymphomaSimon D. Wagner and Jaspal S. KaedaAntibody Techniques Used in the Study of Anaplastic Lymphoma Kinase-Positive ALCLKaren Pulford, Helen M. Roberton, and Margaret JonesIdentification of Anaplastic Lymphoma Kinase Variant Translocations Using 5'RACELuis Hernandez and Elias CampoFollicular Lymphoma: Quantitation of Minimal Residual Disease by PCR of the t(14;18) TranslocationAngela Darby and John G. GribbenIndex