Description
Oxford MHC Volume 1 A Practical Approach 2002 Edition by N. Fernandez, G. Butcher
The major histocompatibility complex is the subject of much research in the immunology area. There is great interest in the structure of MHC proteins and in their function as antigen presenting molecules. Many immunology labs are now faced with the prospect of tackling biochemical and genetic techniques in order to study these molecules, and these two Practical Approach volumes provide the ideal introduction. MHC 1, Antigen Processing and Presentation, iscomplemented by MHC 2, Genes, Cells, and Molecules. Table of contents :- 1. Isolation and functional properties of antigen presenting cells ; 2. Labelling methods for analysis of Class II MHC endocytosis and peptide turnover ; 3. Methods for detecting signalling via MHC class II molecules which can lead to either activation or programmed cell death ; 4. Peptide translocation into the ER ; 5. The 20S proteasome and antigen presentation ; 6. Analysis of MHC class II specific T cell clones ; 7. Methods to study the role of the MHC in NK toxicity ; 8. The interaction of superantigens with MHC class II molecules ; 9. Methods for the generation of T cell clones and epithelial cell lines from excised human biopsies or needle aspirates ; 10. The application of mass spectrometry to the analysis of peptides bound to MHC ; 11. The molecular basis of alloreactivity ; 12. Elution and analysis of peptide pools from MHC class I molecules