Description
Springer Marijuana And Cannabinoid Research Methods And Protocols by Emmanuel S. Onaivi
A cutting-edge collection of readily reproducible in vitro and in vivo methods to elucidate the mechanisms associated with cannabinoid function in health and disease. The techniques can be used in studies across the board from genes to behavior. The molecular neurobiological methods are invaluable in analyzing the structure, the polymorphisms, and the molecular expression of the cannabinoid receptors (CBrs), as well as their association with polysubstance abuse. There are also methods for localizing cannabinoid receptors in different systems, visualizing cannabinoid effects using brain slice imaging and elctrophysiological approaches, and designing and synthesizing cannabinoids and endocannabinoids. The protocols follow the successful Methods in Molecular Medicine (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls _x000D_Molecular Neurobiological Methods in Marijuana-Cannabinoid Research_x000D_
George R. Uhl, Hiroki Ishiguro, Emmanuel S. Onaivi, Zhicheng Lin, B. Emmanuel Akinshola, Bruce Hope, Claire M. Leonard, and Qing-Rong Liu_x000D_
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Experimental Methods to Study the Role of the Peripheral Cannabinoid Receptor in Immune Function_x000D_
Nancy E. Buckley, Diep Burbridge, Manop Buranapramest, Tanya Ferguson, and Renee Y. Paau_x000D_
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Localization of Cannabinoid Receptors Using Immunoperoxidase Methods_x000D_
Guy A. Cabral_x000D_
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Localization of Cannabinoid CB1 Receptor mRNA Using Ribonucleotide Probes: Methods for Double- and Single-Label In Situ Hybridization_x000D_
Andrea G. Hohmann_x000D_
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Morphometric Study on Cytoskeletal Components of Neuronal and Astroglial Cells After a Chronic CB1 Agonist Treatment_x000D_
Patricia Tagliaferro, Alberto J. Ramos, Emmanuel S. Onaivi, Sergio G. Evrard, Maite Duhalde Vega, and Alicia Brusco_x000D_
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Visualizing Cannabinoid Effects Using Brain Slice Imaging and Electrophysiological Approaches_x000D_
Alexander F. Hoffman and Carl R. Lupica_x000D_
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Methods for the Synthesis of Cannabinergic Ligands_x000D_
Ganesh A. Thakur, Spyros P. Nikas, Richard I. Duclos, Jr., and Alexandros Makriyannis_x000D_
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Cannabinoid Receptor Binding to Membrane Homogenates and Cannabinoid-Stimulated [35S]GTPgS Binding to Membrane Homogenates or Intact Cultured Cells_x000D_
Chris Breivogel_x000D_
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Methods to Assay Anandamide Hydrolysis and Transport in Synaptosomes_x000D_
Filomena Fezza, Natalia Battista, Monica Bari, and Mauro Maccarrone_x000D_
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Methods Evaluating Cannabinoid and Endocannabinoid Effects on Gastrointestinal Functions_x000D_
Nissar A. Darmani_x000D_
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The Bioassay of Cannabinoids Using the Mouse Isolated Vas Deferens_x000D_
Ad`ele Thomas and Roger G. Pertwee_x000D_
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Assessment of Cannabis Craving Using the Marijuana Craving Questionnaire_x000D_
Stephen J. Heishman and Edward G. Singleton_x000D_
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Methods for Studying Acute and Chronic Effects ofMarijuana on Human Associative Processes and Memory_x000D_
Robert I. Block_x000D_
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Methods for Clinical Research Involving Cannabis Administration_x000D_
David A. Gorelick and Stephen J. Heishman_x000D_
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Neurological Assessments of Marijuana Users_x000D_
Jean Lud Cadet, Karen Bolla, and Ronald I. Herning_x000D_
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Behavioral Methods in Cannabinoid Research_x000D_
Ester Fride, Alex Perchuk, F. Scott Hall, George R. Uhl, and Emmanuel S. Onaivi_x000D_
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Methods to Study the Behavioral Effects and Expression of CB2 Cannabinoid Receptors and Its Gene Transcripts in Chronic Mild Stress Model of Depression_x000D_
Emmanuel S. Onaivi, Hiroki Ishiguro, Patel Sejal, Paul A. Meozzi, Lester Myers, Patricia Tagliaferro, Bruce Hope, Claire M. Leonard, George R. Uhl, Alicia Brusco, and Eileen Gardner_x000D_
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Index_x000D_