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Humana Press Mast Cells Methods And Protocols by Guha Krishnaswamy , David S. Chi
A cutting-edge collection of readily reproducible techniques for the isolation, culture, and study of activation and signaling in human mast cells. These methods take advantage of the latest advances in molecular biology, technology, and information science. They include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis.show more Part I. Overview of Mast Cell BiologyPaul Ehrlich's 'Mastzellen'-From Aniline Dyes to DNA Chip Arrays: A Historical Review of Developments in Mast Cell ResearchHarsha Vyas and Guha KrishnaswamyThe Human Mast Cell: An OverviewGuha Krishnaswamy, Omar Ajitawi, and David S. ChiMast Cells in Allergy and Autoimmunity: Implications for Adaptive ImmunityGregory D. Gregory and Melissa A. BrownPart II. Identification of Mast Cells in Culture and in TissueAnalysis of MCT and MCTC Mast Cells in TissueLawrence B. SchwartzMast Cell Ultrastructure and Staining in TissueShruti A. Shukla, Ranjitha Veerappan, Judy S. Whittimore, Lou Ellen Miller, and George A. YoungbergExpression of Cell Surface Antigens on Mast Cells: Mast Cell PhenotypingAlexander W. Hauswirth, Stefan Florian, Gerit-Holger Schernthaner, Maria-Theresa Krauth, Karoline Sonneck, Wolfgang R. Sperr, and Peter ValentIdentification of Mast Cells in the Cellular Response to Myocardial InfarctionNikolaos G. Frangogiannis and Mark L. EntmanPart III. Development of Mast Cells In VitroGrowth of Human Mast Cells From Bone Marrow and Peripheral Blood-Derived CD34+ Pluripotent Progenitor CellsArnold S. Kirshenbaum and Dean D. MetcalfeCulture of Human Mast Cells From Hemopoietic ProgenitorsHirohisa SaitoIsolation, Culture, and Characterization of Intestinal Mast CellsGernot Sellge and Stephan C. BischoffPart IV. Mast Cell Signaling and Gene ExpressionActivation of Nuclear Factor-kBChuanfu Li, Jim Kelley, and Tuanzhu HaAnalysis of Mitogen-Activated Protein Kinase ActivationStephen C. ArmstrongMicroarray and Gene-Clustering AnalysisTsung-Hsien Tsai, Denise M. Milhorn, and Shau-Ku HuangTechniques to Study FceRI SignalingYuko Kawakami, Jiro Kitaura, and Toshiaki KawakamiPart V. Mast Cell Expression and Release of Inflammatory MediatorsHuman Mast Cell Proteases: Activity Assays Using Thiobenzyl Ester SubstratesDavid A. JohnsonMast Cell Histamine and Cytokine AssaysDavid S. Chi, S. Matthew Fitzgerald, and Guha KrishnaswamyMeasurement of Mast Cell Cytokine Release by Multiplex AssayKevin F. Breuel and W. Keith De PontiAssays for Histamine-Releasing Factors: From Identification and Cloning to Discovery of Binding PartnersJacqueline M. Langdon and Susan M. MacDonaldAssays for Nitric Oxide ExpressionWilliam L. Stone, Hongsong Yang, and Min QuiImmunohistological Detection of Growth Factors and Cytokines in Tissue Mast CellsZhenhong QuPart VI. Mast Cell Interactions With Other Cell TypesEndothelial Cell Activation by Mast Cell MediatorsKottarappat N. Dileepan and Daniel J. StechschulteCo-Culture of Mast Cells With Fibroblasts: A Tool to Study Their CrosstalkIdo Bachelet, Ariel Munitz, and Francesca Levi-SchafferDetection of e Class Switching and IgE Synthesis in Human B CellsJerome Pene, Florence Guilhot, Isabelle Cognet, Paul Guglielmi, Angelique Guay-Giroux, Jean-Yves Bonnefoy, Greg C. Elson, Hans Yssel, and Jean-Francois GauchatPart VII. Novel Aspects of Mast Cell Activation and RegulationGene Silencing Using Small Interference RNA in Mast CellsDeling Yin and Charles A. StuartMast Cell Activation by LipoproteinsJim Kelley, Gregory Hemontolor, Walid Younis, Chuanfu Li, Guha Krishnaswamy, and David S. ChiMast Cell Activation by StressAnn L. BaldwinPart VIII. Roles of Mast Cells in Host DefenseIn Vivo Models for Studying Mast Cell-Dependent Responses to Bacterial InfectionChristopher P. Shelburne, James B. McLachlan, and Soman N.