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Humana Press Medical Biomethods Handbook by John M. Walker , Ralph Rapley
John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.show more Basic Techniques in Molecular Biology Ralph Rapley Probe Design, Production, and Applications Marilena Aquino de Muro Restriction Enzymes: Tools in Clinical Research Gareth J. S. Jenkins Southern Blotting as a Diagnostic Method Bronwen Harvey and Pirkko Soundy Western Blotting as a Diagnostic Method Pirkko Soundy and Bronwen Harvey Principles and Medical Applications of the Polymerase Chain Reaction Bimal D. M. Theophilus Single-Strand Conformation Polymorphism (SSCP) Analysis Igor Vorechovsky Denaturing Gradient Gel Electrophoresis (DGGE) Jeroen H. Roelfsema and Dorien J. M. Peters Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Sequencing Anu Suomalainen and Ann-Christine Syvanen Introduction to Capillary Electrophoresis of DNA: Biomedical Applications Beatriz Sanchez-Vega Mapping Techniques Simon G. Gregory Dideoxyfingerprinting for Mutation Detection Ioannis Bossis, Antonios Voutetakis, and Constantine A. Stratakis Conformation-Sensitive Gel Electrophoresis Marian Hill Amplification Refractory Mutation System and Molecular Diagnostics Richard Kitching and Arun Seth Ligase Chain Reaction Carla Osiowy Chemical Cleavage of Mismatch: Theory and Clinical Applications Neil V. Whittock and Louise Izatt The Protein Truncation Test Johan T. den Dunnen Linkage, Allele Sharing, and Association Mara Giordano Single-Nucleotide Polymorphisms: Technology and Applications Cyril Mamotte, Frank Christiansen, and Lyle J. Palmer cDNA Microarrays Phillip G. Febbo Nucleic Acid Sequence-Based Amplification Katherine Loens, D. Ursi, H. Goosens, and M. Ieven The Oligonucleotide Ligation Assay Faye A. Eggerding QuantitativeTaqMan Real-Time PCR: Diagnostic and Scientific Applications Joerg Doetsch, Ellen Schoof, and Wolfgang Rascher Use of Denaturing High-Performance Liquid Chromatography in Molecular Medicine Elizabeth L. Rugg and Gareth J. Magee Quantitative PCR David Sugden Liquid Chromatography-Mass Spectrometry of Nucleic Acids Herbert Oberacher and Walther Parson Comparative Genomic Hybridization in Clinical and Medical Research Peng-Hui Wang, Yann-Jang Chen, and Chi-Hung Lin Bioinformatics Tools for Gene and Protein Sequence Analysis Bernd H. A. Rehm and Frank Reinecke In Situ Hybridization Amanda D. Watters Enzyme-Linked Immunosorbent Assay William J. Jordan Protein Therapeutics: Mouse, Humanized, and Human Antibodies Benny K. C. Lo Karyotyping Avery A. Sandberg, John F. Stone, and Zhong Chen Microsatellite Analysis Rachel E. Ibbotson and Anton E. Parker Analysis of Chromosomal Translocations Andreas Hochhaus Differential Display: Theory and Applications Irina Gromova, Pavel Gromov, and Julio E. Celis Techniques for Gene Expression Profiling Mark P. Richards Capillary Electrophoresis in Clinical Analysis Margaret A. Jenkins and Sujiva Ratnaike Flow Cytometry in the Biomedical Arena James L. Weaver and Maryalice Stetler-Stevenson Immunocytochemistry Patricia A. Fetsch Ribotyping in Clinical Microbiology Patricia Severino and Sylvain Brisse Diagnostic Applications of Protein Microarrays Samir Hanash The Human Genome Project Rahul Chodhari and Eddie Chung Prenatal Diagnosis of Inborn Errors of Metabolism Guy T. N. Besley Gene Therapy: Methods and Application Stella B. Somiari Index