Description
Humana Membrane Transporters Methods And Protocols by Qing Yan
Studies of membrane transporters have had great impact on our und- standing human diseases and the design of effective drugs. About 30% of current clinically marketed drugs are targeting membrane transporters or channels. Membrane Transporters: Methods and Protocols provides various practical methodologies for the ongoing research on membrane transporters. To provide readers the most up-to-date information, several emerging fields and methodologies are embraced in this book, including pharmacogenomics, bioin-formatics, and microarray technology. Pharmacogenomics studies of membrane transporters are useful in drug discovery and in predicting drug responses in the clinic. In this volume, the current status of pharma- genomics studies of transporters is reviewed and research methodologies in this field are described. Transporter classification is important in studying the structure and function of membrane transporters and has thus triggered intensive interest in recent years. Membrane Transporters: Methods and Protocols provides a systematic classification of all transmembrane transport proteins found in living org- isms on Earth. This classification system will be helpful for further studies on various aspects of membrane transporters, especially for such large-scale gene expression studies as those employing microarray technologies. Bioinformatics is frequently used in transporter studies and has become indispensable for all kinds of research methods. Commonly used bio- formatics methods, such as databases and tools for sequence analysis and - tif studies, are explained in order to facilitate membrane transporters research. Because of heterogeneous sources and tremendous amounts of data, data in- gration has become one of the most important issues in transporter studies. Pharmacogenomics of Membrane Transporters: An OverviewQing YanThe IUBMB-Endorsed Transporter Classification SystemWolfgang Busch and Milton H. Saier, Jr.Bioinformatics and Data Integration in Membrane Transporter StudiesQing YanDNA Microarrays to Analyze Gene Expression in Animals With Altered Transporter ExpressionGary W. MillerMicroarray Data Analysis in Studies of Membrane TransportersDonglei HuLaser Capture Microdissection to Examine Transporter Expression in Specific Cell RegionsOkkyung Rho and Gary W. MillerSite-Directed Mutagenesis in the Study of Membrane TransportersRenae M. Ryan, Ann D. Mitrovic, and Robert J. VandenbergFluorescence Techniques for Studying Membrane Transport Proteins: The P-Glycoprotein Multidrug TransporterFrances J. Sharom, Paula L. Russell, Qin Qu, and Peihua LuReconstitution of Membrane TransportersFrances J. Sharom and Paul D. W. EckfordEquilibrium Binding and Transport StudiesAna M. Ojeda, Dawn T. Bravo, Terry L. Hart, and Stanley M. ParsonsElectrophysiological Studies of Ion Channels and Therapeutic Potential with Adenovirus-Mediated Gene TransferJihong Qu, Thai V. Pham, and Maria N. ObreztchikovaAntibody Production and Immunocytochemical Localization of Amino Acid TransportersDavid V. Pow, Robert Sullivan, and Heather ScottXenopus laevis OocytesStefan BroeerMeasurement of Intracellular pHFrederick B. Loiselle and Joseph R. CaseySmall-Angle X-Ray Scattering Studies of Membrane Transporter Peripheral Components and Soluble DomainsBrian H. ShiltonMembrane Protein NMR StudiesStanley J. OpellaAnalysis of Soluble Sugar Permease Domains by Solution NMRThomas A. Wilkinson and Yuan ChenMolecular Modeling and Molecular Dynamics Simulationsof Membrane Transporter ProteinsDonard S. DwyerImaging Plasma Membrane Proteins by Confocal MicroscopyStella W. Y. Tsang and Peter M. HaneyIndex