Description
Humana Press Microarrays In Clinical Diagnostics by Thomas O. Joos , Paolo Fortina
Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.show more Noncontact Laser Microdissection and Pressure Catapulting: Sample Preparation for Genomic, Transcriptomic, and Proteomic AnalysisYilmaz Niyaz, Monika Stich, Bernd Sagmuller, Renate Burgemeister, Gabriele Friedemann, Ulrich Sauer, Rainer Gangnus, and Karin SchutzeApplications of the Universal DNA Microarray in Molecular MedicineReyna Favis, Norman P. Gerry, Yu-Wei Cheng, and Francis BaranySensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray HybridizationZhi-wei Zhang, Yi-ming Zhou, Yan Zhang, Yong Guo, Sheng-ce Tao, Ze Li, Qiong Zhang, and Jing ChengGenotyping Single-Nucleotide Polymorphisms by Minisequencing Using Tag ArraysLovisa Lovmar and Ann-Christine SyvanenSingle-Nucleotide Polymorphism and Mutation Identification by the Nanogen Microelectronic Chip TechnologyMaurizio Ferrari, Laura Cremonesi, Pierangelo Bonini, Barbara Foglieni, and Stefania StenirriMolecular Diagnostic Testing for Inherited Thrombophilia Using Invader (R)Margaret A. KellerControlled Agitation During Hybridization: Surface Acoustic Waves Are Shaking Up Microarray TechnologyAchim WixforthRapid Screening for 31 Mutations and Polymorphisms in the Cystic Fibrosis Transmembrane Regulator Gene by Luminex (R) xMAP (TM) Suspension ArraySherry A. Dunbar and James W. JacobsonProtein Microarray-Based Screening of Antibody SpecificityRhonda Bangham, Gregory A. Michaud, Barry Schweitzer, and Paul F. PredkiMultiplexed Protein Analysis Using Antibody Microarrays and Label-Based DetectionBrian B. HaabAllergen MicroarraysTito Bacarese-Hamilton, Julian Gray, Andrea Ardizzoni, and Andrea CrisantiEnhanced Protein Profiling Arrays for Quantitative Measurement of Protein Expression in Multiple SamplesRuochun Huang, Qian Shi,Weimin Yang, and Ruo-Pan HuangMultiplexed Cytokine Sandwich Immunoassays: Clinical ApplicationsUma Prabhakar, Edward Eirikis, Bruce E. Miller, and Hugh M. DavisValidation and Quality Control of Protein Microarray-Based Analytical MethodsLarry J. Kricka and Stephen R. MasterTissue MicroarraysRonald Simon, Martina Mirlacher, and Guido SauterIndex