Description
Springer Microorganisms And Bioterrorism by Burt Anderson, Herman Friedman
The threat of bioterrorism has become a major challenge for the twenty-?rst century. However, the potentials of infectious agents as bioweapons have been recognized for centuries. Throughout history there have been attempts to i- tiate infectious disease outbreaks and epidemics during warfare. In the last decade the attention of the biomedical community, as well as governments and the United Nations, has increasingly focused on the threat of bioterr- ism, especially the use of biological and/or chemical weapons against military and civilian populations. As an example, there is now much interest conce- ing microbial infection and bioterrorism in the medical microbiology and - munologycommunities. Thisvolumeaddressessuchconcernsandemphasizes bothbasicandclinicalconcepts,aswellasproblematicimplicationsofinfection by various microbes now recognized as potential bioterrorism agents. The ?rst chapter by Drs. Andrew Canons, Philip Amuso, and Burt And- son from the University of South Florida is an overview of the biotechnology of bioterrorismbothinthepublichealthresponsetopossibleactsofbioterrorism, aswellasfortheconcernsaboutthemisuseofbiotechnology.Thesecondch- ter is a historical perspective of microbial bioterrorism by Dr. Steven Morse, Director of the Bioterrorism Division at the Center for Disease Control and Prevention in Atlanta, GA. This chapter describes in detail historical aspects concerning the early use of biological agents in warfare, development and international conventions to prohibit the use of such weapons, and a brief - scription of important incidents of infectious agents as bioterrorist agents and use during the last few centuries. The next chapter by Dr. Sandra Gompf from the University of South Florida discusses the role of public health physicians and infectious diseases specialists in the control of microbial bioterrorism. Part I. The Organism, Community Resources, and Genome-wide StudiesThe Secret Lives of DictyosteliumRichard H. KessinThe Genome of Dictyostelium discoideumAdam Kuspa and William F. LoomisThe cDNA Sequencing ProjectHideko Urushihara, Takahiro Morio, and Yoshimasa TanakadictyBase and the Dicty Stock CenterPetra Fey, Pascale Gaudet, Karen E. Pilcher, Jakob Franke, and Rex L. ChisholmAnalysis of Gene Expression Using cDNA MicroarraysMarcel Kaul and Ludwig EichingerProteomic Analysis of Dictyostelium discoideumUdo Roth, Stefan Muller, and Franz-Georg HanischPart II. Basic Methods and Molecular Genetic TechniquesCultivation, Spore Production, and MatingHideko UrushiharaParasexual Genetics Using Axenic CellsJason King and Robert InsallSlug Phototaxis, Thermotaxis, and Spontaneous Turning BehaviorPaul R. Fisher and Sarah J. AnnesleyPurification Techniques of Subcellular Compartments for Analytical and Preparative PurposesLaurence Aubry and Gerard KleinGeneration of Multiple Knockout Mutants Using the Cre-loxP SystemAlan R. Kimmel and Jan FaixRestriction Enzyme-Mediated Integration (REMI) MutagenesisAdam KuspaRNA Interference and Antisense-Mediated Gene Silencing in DictyosteliumMarkus Kuhlmann, Blagovesta Popova, and Wolfgang NellenPart III. Imaging and Localization MethodsApplication of Fluorescent Protein Tags as Reporters in Live-Cell Imaging StudiesAnnette Muller-TaubenbergerInvestigating Gene Expression: In Situ Hybridization and Reporter GenesRicardo Escalante and Leandro SastreApplication of 2D and 3D DIAS to Motion Analysis of Live Cells in Transmission and Confocal Microscopy ImagingDeborah Wessels, Spencer Kuhl, and David R. SollUsingQuantitative Fluorescence Microscopy and FRET Imaging to Measure Spatiotemporal Signaling Events in Single Living CellsXuehua Xu, Joseph A. Brzostowski, and Tian JinVisualizing Signaling and Cell Movement During the Multicellular Stages of Dictyostelium DevelopmentDirk Dormann and Cornelis J. WeijerUnder-Agarose Chemotaxis of Dictyostelium discoideumDavid Woznica and David A. KnechtOptimized Fixation and Immunofluorescence Staining Methods for Dictyostelium CellsMonica Hagedorn, Eva M. Neuhaus, and Thierry SoldatiCryofixation Methods for Ultrastructural Studies of Dictyostelium discoideumMark J. Grimson and Richard L. BlantonPart IV. Dictyostelium as Model OrganismAnalysis of Signal Transduction: Formation of cAMP, cGMP, and Ins(1,4,5)P3 In Vivo and In VitroPeter J. M. Van HaastertAssaying Chemotaxis of Dictyostelium CellsMichelle C. Mendoza and Richard A. FirtelCharacterization of Cross-Linked Actin Filament Gels and Bundles Using Birefringence and Polarized Light ScatteringRuth Furukawa and Marcus FechheimerQuantitative and Microscopic Methods for Studying the Endocytic PathwayFrancisco Rivero and Markus ManiakPreparation of Intact, Highly Purified Phagosomes from DictyosteliumDaniel Gotthardt, Regis Dieckmann, Vincent Blancheteau, Claudia Kistler, Frank Reichardt, and Thierry SoldatiAssaying Cell-Cell AdhesionSalvatore BozzaroPeriodic Activation of ERK2 and Partial Involvement of G Protein in ERK2 Activation by cAMP in Dictyostelium CellsMineko MaedaAn Improved Method for Dictyostelium Centrosome IsolationIrene Schulz, Yvonne Reinders, Albert Sickmann, and Ralph GrafEpigenetics in DictyosteliumMarkus Kaller, Wolfgang Nellen, and Jonathan R. ChubbDictyostelium discoideum as a Model to Study