Description
Humana Press Inc. Microtubule Protocols by Jun Zhou
This book presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs. The book contains a straightforward presentation of readily reproducible protocols, tips for troubleshooting, and advice on avoiding common mistakes. Basic scientists and clinical researchers will benefit from this collection._x000D_ _x000D_
Microtubule Protocols_x000D_
Editor: Zhou, Jun (Nankai University, Tianjin, China)_x000D_
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Table of Contents:_x000D_
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Chapter 1 (review)_x000D_
Microtubules: an overview_x000D_
Richard H. Wade_x000D_
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Chapter 2_x000D_
Large scale purification of brain tubulin with the modified Weisenberg procedure_x000D_
Jose Manuel Andreu_x000D_
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Chapter 3_x000D_
Purification and mass-spectrometry identification of microtubule-binding proteins from Xenopus egg extracts_x000D_
Vincent Gache, Patrice Waridel, Sylvie Luche, Andrej Shevchenko and Andrei V. Popov_x000D_
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Chapter 4_x000D_
Preparation and characterization of post-translationally modified tubulins from Artemia Franciscana_x000D_
Paul A. O'Connell and Thomas H. MacRae_x000D_
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Chapter 5_x000D_
Studying the structure of microtubules by electron microscopy_x000D_
Linda A. Amos and Keiko Hirose_x000D_
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Chapter 6_x000D_
Fluorescence microscopy of microtubules in cultured cells_x000D_
Irina Semenova and Vladimir Rodionov_x000D_
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Chapter 7 (review)_x000D_
Measurements of stathmin-tubulin interaction in solution_x000D_
Marie-France Carlier_x000D_
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Chapter 8_x000D_
Analysis of microtubule dynamics by polarized light_x000D_
Rudolf Oldenbourg_x000D_
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Chapter 9_x000D_
Visualization of spindle behavior using confocal microscopy_x000D_
Adam I. Marcus_x000D_
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Chapter 10_x000D_
Live cell approaches for studying kinetochore-microtubule interactions in Drosophila_x000D_
Daniel W. Buster and David J. Sharp_x000D_
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Chapter 11_x000D_
Analysis of tubulin transport in nerve processes_x000D_
Andrey Tsvetkov and Sergey Popov_x000D_
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Chapter 12_x000D_
Analysis of microtubule-mediated intracellular viral transport_x000D_
Chunyong Liu, Min Liu and Jun Zhou_x000D_
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Chapter 13_x000D_
Xenopus oocyte wound healing as a model system for analysis of microtubule-actin interactions_x000D_
Tong Zhang and Craig A. Mandato_x000D_
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Chapter 14_x000D_
Screening for inhibitors of microtubule-associated motor proteins_x000D_
Frank Kozielski, Salvatore DeBonis and DimitriosSkoufias_x000D_
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Chapter 15 (review)_x000D_
Clinical pharmacology and use of microtubule-targeting agents in cancer therapy_x000D_
Amelia B. Zelnak_x000D_
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Chapter 16_x000D_
Studying drug-tubulin interactions by X-ray crystallography_x000D_
Audrey Dorleans, Marcel Knossow and Benoit Gigant_x000D_
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Chapter 17_x000D_
Characterizing ligand-microtubule binding by competition methods_x000D_
Jose Fernando Diaz and Ruben Martinez Buey_x000D_
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Chapter 18_x000D_
Methods for studying vinca alkaloid interactions with tubulin_x000D_
Sharon Lobert and John J. Correia_x000D_
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Chapter 19_x000D_
High-throughput screening of microtubule-interacting drugs_x000D_
Susan L. Bane, Rudravajhala Ravindra and Anna A. Zaydman_x000D_
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Chapter 20 (review)_x000D_
Strategies for the development of novel taxol-like agents_x000D_
Susan L. Mooberry_x000D_