Description
Humana Press Molecular Analysis of Cancer 1st Editon 2013 Softbound by Jacqueline Boultwood, Carrie Fidler
Over the past 20 years, technological advances in molecular biology have proven invaluable to the understanding of the pathogenesis of human cancer. The application of molecular technology to the study of cancer has not only led to advances in tumor diagnosis, but has also provided markers for the assessment of prognosis and disease progression. The aim of Molecular Ana- sis of Cancer is to provide a comprehensive collection of the most up-to-date techniques for the detection of molecular changes in human cancer. Leading researchers in the field have contributed chapters detailing practical pro- dures for a wide range of state-of-the-art techniques. Molecular Analysis of Cancer includes chapters describing techniques for the identification of chromosomal abnormalities and comprising: fluor- cent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), and microsatellite analysis. FISH has a pro- nent role in the molecular analysis of cancer and can be used for the detection of numerical and structural chromosomal abnormalities. The recently described SKY, in which all human metaphase chromosomes are visualized in specific colors, allows for the definition of all chromosomal rearrangements and marker chromosomes in a tumor cell. Protocols for the detection of chromosomal re- rangements by PCR and RT-PCR are described, as well as the technique of DNA fingerprinting, a powerful tool for studying somatic genetic alterations in tumorigenesis. Molecular Analysis of Cancer.- Detection of Chromosome Abnormalities in Leukemia Using Fluorescence In Situ Hybridization.- Spectral Karyotyping in Cancer Cytogenetics.- Comparative Genomic Hybridization Analysis.- Detection of Chromosomal Deletions by Microsatellite Analysis.- Detection and Quantification of Leukemia-Specific Rearrangements.- Detection of t(2;5)(p23;q35)Translocation by Long-Range PCR of Genomic DNA.- Use of DNA Fingerprinting to Detect Genetic Rearrangements in Human Cancer.- Mutation Analysis of Large Genomic Regions in Tumor DNA Using Single-Strand Conformation Polymorphism.- Mutational Analysis of Oncogenes and Tumor Suppressor Genes in Human Cancer Using Denaturing Gradient Gel Electrophoresis.- Detection of Mutations in Human Cancer Using Nonisotopic RNase Cleavage Assay.- Mutational Analysis of the Neurofibromatosis Type 1 Gene in Childhood Myelodysplastic Syndromes Using a Protein Truncation Assay.- Mutation Analysis of Cancer Using Automated Sequencing.- Detection of Differentially Expressed Genes in Cancer Using Differential Display.- Genomewide Gene Expression Analysis Using cDNA Microarrays.- Gene Expression Profiling in Cancer Using cDNA Microarrays.- Wilms Tumor Gene WT1 as a.- Detection of Aberrant Methylation of the p15 INK4B Gene Promoter.- Clonality Studies in Cancer Based on X Chromosome Inactivation Phenomenon.- 20Telomere Length Changes in Human Cancer.- Measurement of Telomerase Activity in Human Hematopoietic Cells and Neoplastic Disorders.