Description
Humana Press Molecular Beacons Signalling Nucleic Acid Probes Methods and Protocols 1st Editon 2010 Softbound by Andreas Marx, Oliver Seitz
Significant DNA sequence data has been made available by several important genome projects. The allocation of functions to a mere string of the four letters in the code of life plays a major if not predominant role of ongoing and future research. Guided by experts in the field, Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols, provides easy-to-follow experiments, commences with an introduction to the basic principles of fluorescence, and shows a diverse set of instructive examples, from probe design to applications in clinical settings, molecular beacon technology, the identification of transgenic mice, along with the potential of realtime PCR. While originally being used for fluorogenic nucleic acid probes with a stem-loop structure, molecular beacon now refers to a whole family of fluorescence-up probes. Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols summarizes all the latest findings and explores powerful techniques designed for geneticist, biotechnologists, molecular and cellular biologists, researchers and developers. Basic Principles of Fluorescence and Energy Transfer.- Applications.- Multiplex Detection of Mutations.- Detection of Salmonella Species in Foodstuffs.- Identification of Homozygous Transgenic Mice by Genomic Real-Time PCR.- Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma.- Absolute Quantitation of Feline Leukemia Virus Proviral DNA and Viral RNA Loads by TaqMan® Real-time PCR and RT-PCR.- High-Throughput Real-Time PCR.- Design and Use of Scorpions Fluorescent Signaling Molecules.- Hybridization Probe Pairs and Single-Labeled Probes: an Alternative Approach for Genotyping and Quantification.- New Technologies.- EasyBeacons™ for the Detection of Methylation Status of Single CpG Duplets.- Quenched Autoligation Probes.- HyBeacon® Probes for Rapid DNA Sequence Detection and Allele Discrimination.- FIT-Probes in Real-Time PCR.- SNP Genotyping by Unlabeled Probe Melting Analysis.- Non-Nucleic Acid Targets.- Molecular Beacons for Protein—DNA Interaction Studies.- DNA Polymerase Profiling.- Modular Reporter Hairpin Ribozymes for Analyzing Molecular Interactions.- Screening of Molecular Interactions Using Reporter Hammerhead Ribozymes.