Description
Humana Molecular Diagnosis Of Cancer 2Ed Methods And Protocols by Joseph E. Roulston , John M. S. Bartlett
We are currently experiencing a fundamental shift in the way in which we approach the characterization of cancer. Never before has the make up of cancer tissues and individual cells been so exhaustively researched and char- terized. We are now capable of producing molecular "fingerprints" that ch- acterize the expression of all known and unknown genes within tumors and their surrounding tissues. More than 30,000 different genes may be measured in each patient's tumor in a single experiment. Simultaneously, novel therapies that exploit the molecular roadmap have been developed and are now being offered to patients. These novel agents, such as Glivec, Herceptin, Iressa, and others, specifically target individual genes within tumors and can produce d- matic responses in some patients. These drugs are only the forerunners of a coming tidal wave of novel therapeutics that individually target specific m- ecules within cancer cells-more than 300 such agents are currently in phase I or II clinical trials. This is an exciting time for cancer specialists and patients alike. However, if we have learned anything from the past 50 or more years of research into cancer, it is that Lord Beaverbrook, in founding the British national health service in the 1950s, was frighteningly prescient when he defined the primary goal of health care to be "Diagnosis, Diagnosis, Diag- sis. " Now, more than ever, it is essential that appropriate diagnostic methods and approaches are applied to the selection of patients for treatment. Prognostic and Predictive FactorsMichael Scott and Peter A. HallAssessment of Predictive Values of Tumor MarkersJoseph E. RoulstonQuality Assurance of Predictive Markers in Breast CancerAnthony Rhodes and Diana M. BarnesExtraction of Nucleic Acid TemplatesJohn M. S. Bartlett and Helen SpeirsMicrodissection and Extraction of DNA From Archival TissueJoanne Edwards, James J. Going, and John M. S. BartlettFluorescence In Situ Hybridization: Technical OverviewJohn M. S. BartlettHER2 FISH in Breast CancerJohn M. S. Bartlett and Amanda ForsythFluorescence In Situ Hybridization for BCR-ABLMark W. Drummond, Elaine K. Allan, Andrew Pearce, and Tessa L. HolyoakeUroVysion (TM) Multiprobe FISH in Urinary CytologyLukas Bubendorf and Bruno GrilliChromogenic In Situ Hybridization in Tumor PathologyJorma Isola and Minna TannerComparative Genomic Hybridization and Fluorescence In Situ Hybridization in Chronic Lymphocytic LeukemiaMarie JarosovaMolecular Characterization of Human Papillomaviruses by PCR and In Situ HybridizationSuzanne D. Vernon and Elizabeth R. UngerA Nested RT-PCR Assay to Detect BCR/ablLinda M. WassermanTP53 Mutation Detection by SSCP and SequencingJenni Hakkarainen, Judith A. Welsh, and Kirsi H. VahakangasPCR Diagnosis of T-Cell Lymphoma in Paraffin-Embedded Bone Marrow BiopsiesJean Benhattar and Sandra GebhardCirculating DNA Analysis: Protocols and Clinical Applications Using Taqman AssaysKwan-Chee Allen Chan and Yuk-Ming Dennis LoMicrosatellite Instability: Theory and MethodsGillian Gifford and Robert BrownThe Diagnostic and Prognostic Significance of the Methylation Status of Myf-3 in Lymphoproliferative DisordersJeremy M. E. Taylor, Peter H. Kay, andDominic V. SpagnoloQuantitative Analysis of PRAME for Detection of Minimal Residual Disease in LeukemiaMaiko Matsushita, Rie Yamazaki, and Yutaka KawakamiDetermination of Cyclin D1 Expression by Quantitative Real-Time, Reverse-Transcriptase Polymerase Chain ReactionKaren E. Bijwaard and Jack H. LichyDetection of Telomerase hTERT Gene Expression and Its Splice Variants by RT-PCRW. Nicol Keith and Stacey F. HoareDetection of Telomerase Enzyme Activity by TRAP AssayW. Nicol Keith and Aileen J. MonaghanIdentification of TP53 Mutations in Human Cancers Using Oligonucleotide MicroarraysWen-Hsiang Wen and Michael F. PressDetection of K-ras Mutations by a Microelectronic DNA ChipEvelyne Lopez-Crapez, Thierry Livache, Patrice Caillat, and Daniela ZsoldosMicroarray-Based CGH in CancerEkaterina Pestova, Kim Wilber, and Walter KingTissue MicroarraysRonald Simon, Martina Mirlacher, Guido SauterIndex