Description
Humana Press Inc. Monoclonal Antibodies Methods And Protocols by Maher Albitar
This book examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting. The chapters offer in-depth description for generating mouse and recombinant humanized antibodies, and a comprehensive review of how antibodies are being used in bead-based methods for measuring proteins. This field will continue to expand and provide new and innovative techniques in the laboratory and as a basis that complements targeted therapy._x000D_ _x000D_
Table of Contents_x000D_
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1. Development and Characterization of Mouse Hybridomas._x000D_
2. Recombinant Monoclonal Antibodies._x000D_
3. Soluble Fc Fusion Proteins for Biomedical Research._x000D_
4. The use of the Antibodies in the Diagnosis of Leukemia and Lymphoma by Flow Cytometry._x000D_
5. Quantification of Surface Antigens and Quantitative Flow Cytometry._x000D_
6. CD13 and CD10 expression of granulocytes as markers for the functioning of the immune system. Quantification of the expression of membrane molecules using 1:1 labeled monoclonal antibodies and flow cytometry._x000D_
7. Monitoring Cell Signaling pathways by Quantitative Flow Cytometry._x000D_
8. Antibodies and Immunohistochemical Evaluation for the Diagnosis of Hematologic Malignancies: An Overview _x000D_
9. Bead Based Multi-Analyte Flow Immunoassays: The Cytometric Bead Array (CBA) System._x000D_
10. Cell-Free bead-based Detection of Total and Phosphorylated Proteins in Plasma and Cell Lysate._x000D_
11. Measuring Humanized Antibodies in Plasma of Patients Treated with Antibody-based Therapy using Bead-based Flow Cytometry._x000D_
12. Detection of Chromosome Translocations by Bead-based Flow Cytometry._x000D_
13. Detection of the MDR1 P-Glycoprotein expression and function._x000D_
14. Detection of human antibodies generated against therapeutic antibodies used in tumor therapy._x000D_
15. Measuring Allergen-Specific IgE: Where have we been and where are we going?_x000D_