Description
Taylor & Francis Natural Biomarkers for Cellular Metabolism Biology Techniques and Applications 2014 Edition by Vladimir V. Ghukasyan, Ahmed A. Heikal
From the Lab to Clinical Settings-Advances in Quantitative, Noninvasive Optical DiagnosticsNoninvasive fluorescence imaging techniques, novel fluorescent labels, and natural biomarkers are revolutionizing our knowledge of cellular processes, signaling and metabolic pathways, the underlying mechanisms for health problems, and the identification of new therapeutic targets for drug discoveries. Natural Biomarkers for Cellular Metabolism: Biology, Techniques, and Applications delves into the current state of knowledge on intrinsic fluorescent biomarkers and highlights recent developments in using these biomarkers for the metabolic mapping and clinical diagnosis of healthy and diseased cells and tissues.Autofluorescent Biomarkers for Biomedical DiagnosticsThe book's first section introduces the fundamentals of cellular energy metabolism as well as natural biomarkers within the context of their biological functions. The second section outlines the theoretical and technical background of quantitative, noninvasive, autofluorescence microscopy and spectroscopy methods, including experimental design, calibration, pitfalls, and remedies of data acquisition and analysis. The last two sections highlight advances in biomedical and biochemical applications, such as monitoring stem cell differentiation in engineered tissues and diagnosing cancer and ophthalmic diseases quantitatively and noninvasively.Tailored to Interdisciplinary Researchers Covering cell biology, imaging techniques, and clinical diagnostics, this book provides readers with a complete guide to studying cellular/tissue metabolism under healthy, diseased, and environment-induced stress conditions using natural biomarkers. The book is designed for graduate and advanced undergraduate students, biophysics instructors, medical researchers, and those in pharmaceutical R&D. Table of contents :- Biochemical, Biological, and Biophysical BackgroundMitochondria and Energy Metabolism: Networks, Mechanisms, and Control Ilmo E. HassinenIntracellular Autof luorescent Species: Structure, Spectroscopy, and Photophysics Nobuhiro Ohta and Takakazu NakabayashiAutofluorescence Imaging Techniques: Fundamentals and ApplicationsOne-Photon Autof luorescence Microscopy Narasimhan Rajaram and Nirmala RamanujamAutofluorescence Lifetime Imaging Michael G. Nichols, Kristina Ward, Lyandysha V. Zholudeva, Heather Jensen Smith, and Richard HallworthPolarization Imaging of Cellular Autofluorescence Harshad D. Vishwasrao, Qianru Yu, Kuravi Hewawasam, and Ahmed A. HeikalReal-Time In Vivo Monitoring of Cellular Energy Metabolism Avraham Mayevsky and Efrat Barbiro-MichaelyTryptophan as an Alternative Biomarker for Cellular Energy Metabolism Vinod Jyothikumar, Yuansheng Sun, and Ammasi PeriasamyAlternative Approaches to Optical Sensing of the Redox State Yi YangNatural Biomarkers for Biochemical and Biological StudiesSpatiotemporal Detection of NADH-Linked Enzyme Activities in Single Cell Metabolism V. Krishnan RamanujanNAD(P)H and FAD as Biomarkers for Programmed Cell Death Hsing-Wen WangMonitoring Stem Cell Differentiation in Engineered Tissues Kyle P. Quinn and Irene GeorgakoudiAutofluorescence as a Diagnostic Tool in Medicine and HealthAutofluorescence-Assisted Examination of Cardiovascular System Physiology and Pathology Alzbeta Marcek ChorvatovaAutofluorescence Perspective of Cancer Diagnostics Lin Z. Li and Nannan SunDynamic Imaging of Intracellular Coenzyme Autofluorescence in Intact Pancreatic Islets Alan K. Lam and Jonathan V. RocheleauAutofluorescence Diagnostics of Ophthalmic Diseases Dietrich SchweitzerPathogen Effects on Energy Metabolism in Host Cells Marta Szaszak and Jan RuppIndex