Nuclear Transfer Protocols Cell Reprogramming And Transgenesis by Paul J. Verma , Springer

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Springer Nuclear Transfer Protocols Cell Reprogramming And Transgenesis by Paul J. Verma

Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear transfer. The volume will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis._x000D_ _x000D_Part I. Nuclear Transfer_x000D_ _x000D_ Origin and Progress of Nuclear Transfer In Nonmammalian Animals_x000D_ Marie A. Di Berardino_x000D_ _x000D_ A. Culture and Maintenance of Nuclear Transfer Embryos_x000D_ _x000D_ In Vitro Maturation and Embryo Production in Cattle_x000D_ Marc-Andre Sirard and Karine Coenen_x000D_ _x000D_ Activation of Oocytes After Nuclear Transfer_x000D_ Zoltan Machaty_x000D_ _x000D_ Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos_x000D_ Bjoern Heindryckx, Josiane Van der Elst, and Marc Dhont_x000D_ _x000D_ Maintenance of Pregnancy in Pigs With Limited Viable Embryos_x000D_ Tim King and Paul A. De Sousa_x000D_ _x000D_ Health and Neonatal Care of Bovine Clones_x000D_ Jacqui T. Forsyth and David N. Wells_x000D_ _x000D_ B. Nuclear Transfer Methods_x000D_ _x000D_ Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice_x000D_ Young Gie Chung, Shaorong Gao, and Keith E. Latham_x000D_ _x000D_ Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors_x000D_ Kazuchika Miyoshi, Sezen Arat, and Steven L. Stice_x000D_ _x000D_ A Double Nuclear Transfer Technique for Cloning Pigs_x000D_ Irina A. Polejaeva, Shawn Walker, and Keith Campbell_x000D_ _x000D_ Nuclear Transfer in Nonhuman Primates_x000D_ Shoukhrat M. Mitalipov and Don P. Wolf_x000D_ _x000D_ Cloning of Exotic/Endangered Species: Desert Bighorn Sheep_x000D_ James Buck Williams, Taeyoung Shin, Ling Liu, Gabriela Flores-Foxworth, Juan Romano, Alice Blue-McClendon, Duane Kraemer, and Mark E. Westhusin_x000D_ _x000D_ Handmade Somatic Cell Cloning in Cattle_x000D_ Gabor Vatja, Ian M. Lewis, and R. Tayfur Tecirlioglu_x000D_ _x000D_ C. Transgenesis_x000D_ _x000D_ Genetic Modification of Sheep by Nuclear Transfer With Gene-Targeted Somatic Cells_x000D_ A. John Clark, Sarah Burl, and Chris Denning_x000D_ _x000D_ Transgenesis Using Nuclear Transfer in Goats_x000D_ Anthoula Lazaris, Rebecca Keyston, Costas N. Karatzas, and Carol L. Keefer_x000D_ _x000D_ Part II. Reprogramming Nuclei_x000D_ _x000D_ Nuclear Reprogramming: AnOverview_x000D_ Takashi Tada_x000D_ _x000D_ Expression of Imprinted Genes in Cloned Mice_x000D_ Takashi Kohda, Fumitoshi Ishino, and Atsuo Ogura_x000D_ _x000D_ Nuclear Remodeling Assay in Xenopus Egg Extract_x000D_ Koichi Gonda and Nobuaki Kikyo_x000D_ _x000D_ In Vitro Reprogramming of Nuclei and Cells_x000D_ Anne-Mari Hakelien, Thomas Kuntziger, Kristine G. Gaustad, Anne Marstad, and Philippe Collas_x000D_ _x000D_ Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells_x000D_ Daniele Pralong and Paul J. Verma_x000D_ _x000D_ Gene Expression and Methylation Patterns in Cloned Embryos_x000D_ Christine Wrenzycki, Doris Herrmann, Claudia Gebert, Joseph W. Carnwath, and Heiner Niemann_x000D_ _x000D_ Part III. Concluding Remarks_x000D_ _x000D_ Are There Any Normal Clones?_x000D_ Ian Wilmut_x000D_ _x000D_ Future and Applications of Cloning_x000D_ Alan O. Trounson_x000D_ _x000D_ Index_x000D_