Description
Humana Press Inc. Patch-Clamp Methods And Protocols by Peter Molnar
Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. _x000D__x000D__x000D_The volume includes an extensive theoretical treatise concerning single channel kinetic analysis._x000D_ _x000D_
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Part 1. Pharmacology_x000D_
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1. Pharmacological analysis of recombinant NR1a/2A and NR1a/2B NMDA_x000D_
receptors using whole-cell patch clamp method. Laszlo Fodor and Jozsef_x000D_
Nagy_x000D_
2. Memantine as an example of a fast, voltage-dependent, open channel_x000D_
N-methyl-D-aspartate (NMDA) receptor blocker. Chris G. Parsons and Kate_x000D_
Gilling_x000D_
3. Methods for evaluation of positive allosteric modulators of_x000D_
glutamate AMPA receptors. Edward R. Siuda, Jennifer C. Quirk and Eric_x000D_
S. Nisenbaum_x000D_
4. Automated voltage clamp technique. Andrea Ghetti, Antonio Guia and_x000D_
Jia Xu_x000D_
5. Flip-the-Tip: automated patch clamping based on glass electrodes._x000D_
Michael Fejtl, Uwe Czubayko, Alexander Hummer, Tobias Krauter and_x000D_
Albrecht Lepple-Wienhues_x000D_
6. The Roboocyte: Automated Electrophysiology Based on Xenopus Oocytes._x000D_
Christine Leisgen, Mike Kuester and Christoph Methfessel_x000D_
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Part 2. Physiology_x000D_
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7. Infrared guided laser stimulation as a tool for elucidating the_x000D_
synaptic site of expression of long-term synaptic plasticity. Gerhard_x000D_
Rammes, Mathias Eder, Walter Zieglgansberger and Hans-Ulrich Dodt_x000D_
8. Single-cell RT-PCR, a Technique to Decipher the Electrical,_x000D_
Anatomical and Genetic Determinants of Neuronal Diversity. Maria_x000D_
Toledo-Rodriguez and Henry Markram_x000D_
9. Mechanosensitive Ion Channels investigated simultaneously by_x000D_
Scanning Probe Microscopy and Patch clamp. Matthias G. Langer_x000D_
10. Synaptic Connectivity in Engineered Neuronal Networks. Peter_x000D_
Molnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das and James J._x000D_
Hickman_x000D_
11. Modeling of Action Potential Generation in NG108-15 Cells. Peter_x000D_
Molnar and James J. Hickman_x000D_
12. Whole-cell Voltage-clamp on Skeletal Muscle Fibers with the_x000D_
Silicone-Clamp Technique. Sandrine Pouvreau, Claude Collet, Bruno_x000D_
Allard and Vincent Jacquemond_x000D_
13. Determination of channel properties at the unitary level in adult_x000D_
mammalian isolated cardiomyocytes. RomainGuinamard_x000D_
14. Electrophysiological Properties of Embryonic Stem Cells During_x000D_
Differentiation into Cardiomyocyte-like Cell Types. Antoni C.G. van_x000D_
Ginneken and Arnoud. C. Fijnvandraat_x000D_
15. Hybrid Neuronal Network Studies Under Dynamic Clamp. Alan D. Dorval_x000D_
II, Jonathan Bettencourt, Theoden I. Netoff and John A. White_x000D_
16. Cardiac channelopathies studied with the dynamic action potential_x000D_
clamp technique. Geza Berecki, Jan G. Zegers, Ronald Wilders and Antoni_x000D_
C.G. van Ginneken_x000D_
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Part 3. Biophysics_x000D_
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17. Principles of Single-Channel Kinetic Analysis. Feng Qin *_x000D_
Theoretical chapter_x000D_
18. Use of Xenopus oocytes to measure ionic selectivity of pore-forming_x000D_
peptides and ion channels. Thierry Cens and Pierre Charnet_x000D_
19. Estimation of quantal parameters with multiple-probability_x000D_
fluctuation analysis. Chiara Saviane and Robin Angus Silver_x000D_