Description
Humana Press Inc. Pcr Mutation Detection Protocols by Edited by Bimal D.M. Theophilus Edited by Ralph Rapley
Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new recent and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g. ASO-PCR SSCP DGGE chemical cleavage) deletions (multiplex PCR FISH blotting) non-sense mutations (PTT) and more. The new and exciting techniques of DNA array analysis along with such recently developed experimental methods as conformation-sensitive gel electrophoresis are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.show more