Plant DNA Fingerprinting and Barcoding Methods and Protocols by Nikolaus J. Sucher, James R. Hennell, Maria C. Carles , Humana

Description

Humana Plant DNA Fingerprinting and Barcoding Methods and Protocols by Nikolaus J. Sucher, James R. Hennell, Maria C. Carles

Molecular cloning and DNA-based analysis have become part of every molecular life science laboratory. The rapid adoption of DNA-based techniques has been facilitated by the introduction of the polymerase chain reaction (PCR), which has made cloning and characterization of DNA quick and relatively simple. PCR is virtually part of every variation of the plethora of approaches used for DNA fingerprinting today. Plant DNA Fingerprinting: Methods and Protocols aims to bring together the different currently available genome-based techniques into one repository. This volume contains detailed protocols for the preparation of plant genomic DNA, fingerprinting of plants for the detection of intra-species variations, the use of DNA barcoding, as well as methods for the bioinformatic analysis of data. Also included are several discussions on the broader issues of genome-based approaches in order to provide a sound understanding of the principles of these methods. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls._x000D__x000D__x000D__x000D_Authoritative and easily accessible, Plant DNA Fingerprinting: Methods and Protocols is tailored principally for those who seek to augment their current methods of plant analysis and quality control using genome-based approaches as well as for scientists and researchers in different plant sciences._x000D_ Table of contents : - _x000D_ 1. A Taxonomist's View on Genomic Authentication_x000D_ _x000D_ Bernard R. Baum_x000D_ _x000D_ _x000D_ _x000D_ 2. DNA Fingerprinting, DNA Barcoding and Next Generation Sequencing Technology in Plants_x000D_ _x000D_ Nikolaus J. Sucher, James Hennell, and Maria C. Carles_x000D_ _x000D_ _x000D_ _x000D_ 3. Challenges in the DNA Barcoding of Plant Material_x000D_ _x000D_ Robyn S. Cowan and Michael F. Fay_x000D_ _x000D_ _x000D_ _x000D_ 4. Plant Genetics for Forensic Applications_x000D_ _x000D_ David N. Zaya and Mary V. Ashley _x000D_ _x000D_ _x000D_ _x000D_ 5. DNA Purification from Multiple Sources in Plant Research with Homemade Silica Resins_x000D_ _x000D_ Jian-Feng Li and Jen Sheen_x000D_ _x000D_ _x000D_ _x000D_ 6. Random Amplified Marker Technique for Plants Rich in Polyphenols_x000D_ _x000D_ Tripta Jhang and Ajit Kumar Shasany_x000D_ _x000D_ _x000D_ _x000D_ 7. Amplified Fragment Length Polymorphism (AFLP) - an Invaluable Fingerprinting Technique for Genomic, Transcriptomic, and Epigenetic Studies_x000D_ _x000D_ Ovidiu Paun and Peter Schoenswetter_x000D_ _x000D_ _x000D_ _x000D_ 8. Restriction Fragment Length Polymorphism (RFLP) of the 5S-rRNA-NTS Region: A Rapid and Precise Method for Plant Identification_x000D_ _x000D_ Cinzia Margherita Bertea and Giorgio Gnavi_x000D_ _x000D_ _x000D_ _x000D_ 9. ISSR: A Reliable and Cost-Effective Technique for Detection of DNA Polymorphism_x000D_ _x000D_ Maryam Sarwat_x000D_ _x000D_ _x000D_ _x000D_ 10. Development of Sequence Characterized Amplified Region (SCAR) from RAPD (Random Amplified Polymorphic DNA) Amplicons_x000D_ _x000D_ Kalpana Joshi and Preeti Chavan _x000D_ _x000D_ _x000D_ _x000D_ 11. Plant DNA Barcoding using Plastid Markers_x000D_ _x000D_ Aron J. Fazekas, Maria L. Kuzmina, Steven G. Newmaster, Peter M. Hollingsworth, David L. Erickson, and W. John Kress_x000D_ _x000D_ _x000D_ _x000D_ 12. Authentication of Medicinal Plants by SNP-Based Multiplex PCR_x000D_ _x000D_ Ok Ran Lee, Min-Kyeoung Kim, and Deok-Chun Yang_x000D_ _x000D_ _x000D_ _x000D_ 13. Multiplex PCR Method to Discriminate Artemisia Iwayomogi from other Artemisia Plants_x000D_ _x000D_ Seung-Eun Oh_x000D_ _x000D_ _x000D_ _x000D_ 14. Loop-Mediated Isothermal Amplification (LAMP) for the Detection of Plant Pathogens_x000D_ _x000D_ Lisa I. Ward and Scott J. Harper_x000D_ _x000D_ _x000D_ _x000D_ 15. Genomic DNA Extraction and Barcoding of Endophytic Fungi_x000D_ _x000D_ Patricia L. Diaz, James Hennell, and Nikolaus J. Sucher_x000D_ _x000D_ _x000D_ _x000D_ 16. Using GenBank (R) for Genomic Authentication: A Tutorial_x000D_ _x000D_ James R. Hennell, Paul M. D'agostino, Samiuela Lee, Cheang S. Khoo, and Nikolaus J. Sucher_x000D_