Description
Taylor and Francis Ltd Plant Transformation via Agrobacterium Tumefaciens 1st Edition 2022 Hardbound by Mangena, Phetole
Plant Transformation via Agrobacterium Tumefaciens compiles fundamental and specific information and procedures involving in vitro soybean transformation, which forms the basis for the Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method serves as one of the most preferred, reliable and cost-effective mechanism of transgene expression in both leguminous recalcitrant species and non-legume crops. The technology is favoured due to its simplicity, feasibility and high transformation rates that are so far achieved mostly in monocot plants and a few dicot genotypes. This book provides a comprehensive review of plant transformation which remains necessary for many researchers who are still facing protocol-related hurdles. Among some of the major topics covered in Plant Transformation via Agrobacterium Tumefaciens are the history and discovery of Agrobacterium bacterium, longstanding challenges causing transformation inefficiencies, types and conditions of explants, development of transgenic plants for stress resistance, and the role of transgenic plants on animal/human health, including the environment. Plant Transformation via Agrobacterium Tumefaciens helps the reader to understand how soybean, like many other orphan legume crops, faces the risk of overexploitation which may render the currently available varieties redundant and extinct should its narrow gene pool not improve. Plant transformation serves as a key technique in improving the gene pool, while developing varieties that are drought tolerant, have enhanced nutritional value, pest resistant and reduce the destruction by disease causing microorganims. This book is an essential foundation tool that is available for researchers and students to reinforce the application of Agrobacterium-mediated genetic transformation in soybean. Overview of Genetic Transformation in PlantsPlant Transformation HistoryAgrobacterium TumefaciensGetting Started with In Vitro Soybean TransformationIn Vitro Cultures Commonly used for Plant TransformationCurrent and Longstanding Challenges facing Soybean TransformationAlternative Techniques for Genetic Manipulations via A. TumefaciensMolecular Aspects of Indirect Gene Transfer via A. TumefaciensGenetic Transformation in Other Leguminous CropsTransgenic Plants for Biotic Stress ResistanceTransgenic Plants for Abiotic Stress ResistancePotential Health Safety Concerns and Environmental RisksThe Regulatory Management of Transgenic PlantsCurrent and Future Prospects in Genetic EngineeringPhotographic Index of Soybean Transformation Cultures