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SPRINGER Plant Virology Methods And Protocols (Hb 2022) by WANG A.
This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research. Preface...Table of Contents...Contributing Authors...1 In Vivo Detection of Double-Stranded RNA by dRBFC AssayXiaofei Cheng, Yameng Luan, and Aiming Wang2 Developing Rice Mutants using CRISPR/Cas9-Based Genome Editing TechnologyKeyan Xu and Yi Li3 Microscale Thermophoresis Assay: A Powerful Method to Quantify Protein-Nucleic Acid and Protein-Protein InteractionsYu Huang and Yi Li4 Transient Expression-Mediated Gene Silencing in Plants and Suppression of Gene Silencing with Viral Suppressors Fangfang Li and Aiming Wang5 Isolation and Transfection of Plant Mesophyll Protoplasts for Virology Research Zhaoji Dai and Aiming Wang6 Split-Luciferase Complementation for Analysis of Virus-Host Protein InteractionsYan Liang and Zhenghe Li7 Monitoring Virus Intercellular Movement from Primary Infected Cells to Neighboring Cells in Plants Zhaoji Dai and Aiming Wang 8 Simultaneous Determination and Subcellular Localization of Protein-Protein Interactions in Plant Cells using Bimolecular Fluorescence Complementation Assay Ziwei Tang, Mark A. Bernards, and Aiming Wang9 A Powerful Method for Studying Protein-Protein Interactions in Plants: Co-immunoprecipitation (Co-IP) Assay Zhihao Jiang, Meng Yang, Qing Cao, Yongliang Zhang, and Dawei Li10 Membrane and Nuclear Yeast Two-Hybrid SystemsQian Chen and Taiyun Wei11 Tag-Based Pull-Down Assay Shanwu Lyu, Changwei Zhang, Xilin Hou, and Aiming Wang12 Purification of Plasmodesmata-Enriched Fraction for Proteomic AnalysesRongrong He, Mark A. Bernards, and Aiming Wang 13 Purification and Proteomics Analysis of Phloem Tissues from Virus-Infected PlantsAaron Simkowich, Susanne E. Kohalmi, and Aiming Wang 14 Rapid and Specific Purification of Argonaute-Small RNA Complexes from Rice for Slicer Activity Shanshan Zhao and Jianguo Wu15 Silencing Specific Genes in Plants Using Virus-Induced Gene Silencing (VIGS) Vectors Tao Zhou, Laihua Dong, Tong Jiang, and Zaifeng Fan 16 A Versatile Expression Platform in Insects and Cereals Based on a CytorhabdovirusJi-Hui Qiao, Qiang Gao, Ying Zang, Xiao-Dong Fang, and Xian-Bing Wang 17 Production of Virus-Free Chrysanthemum morifolium Ramat by Tissue Culture TechniquesKeru Yan, Xuejie Du, and Bizeng Mao18 Long-Term Preservation of Plant Viruses in Cryopreserved Shoot TipsMin-Rui Wang, Tianxing Pang, Ziqian Lian, Qiao-Chun Wang, and Liying Sun 19 Establishment of White-Backed Planthopper Cell Lines Hongyan Chen, Wei Wu, and Taiyun Wei20 Biolistic Inoculation of Fruit Tress with Full-Length Infectious cDNA Clones of RNA Viruses Yinzi Li and Aiming Wang21 Purification of Total RNAs and Small RNAs from Fruit Tree Leaf TissuesYuxin Ma and Shifang Li22 Diagnosis of Viral Diseases Using Deep Sequencing and Metagenomics AnalysesAli Raza and Qingfa Wu23 Immunocapture-Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay for Detection of Plant RNA VirusesSubhas Hajeri and Raymond Yokomi24 Detection of Tomato spotted wilt virus (TSWV) Infection in Plants using DAS-ELISA and Dot-ELISA Shibo Gao and Jianxiang Wu 25 Tube Capture (TC) RT-PCR and Multiplex RT-PCR for Diagnosis and Characterization of Viruses in Fruit Trees Liping Wang26 Detection of Cucumber green mottle mosaic virus (CGMMV) in Cucurbitaceous Crop Seeds by RT-PCRYi Xie and Jianxiang Wu27 RNA In Situ Hybridization of Detecting Cucumber Mosaic Virus in Shoots of Nicotiana benthamiana Plants Jing-Ze Zou, De-Shui Liu, Xin Tong, Xiao-Peng Zhang, and Xian-Bing Wang28 Transmission Electron Microscopic Methods for Plant Virology Li Xie and Jian Hong Subject Index List...