Description
Humana Platelets & Megakaryocytes Vol.2 by Jonathan M. Gibbins , Martyn P. Mahaut-Smith
12 The average human body has on the order of 10 circulating platelets. They are crucial for hemostasis, and yet excessive platelet activation is a major cause of m- bidity and mortality in Western societies. It is therefore not surprising that platelets have become one of the most extensively investigated biological cell types. We are, however, far from understanding precisely how platelets become activated under physiological and pathophysiological conditions. In addition, there are large gaps in our knowledge of platelet production from their giant precursor cell, the megakar- cyte. Understanding megakaryocyte biology will be crucial for the development of platelet gene targeting. The aim of Platelets and Megakaryocytes is therefore to bring together established and recently developed techniques to provide a comprehensive guide to the study of both the platelet and the megakaryocyte. It consists of five s- tions split between two volumes. The more functional assays appear in Volume 1, whereas Volume 2 includes signaling techniques, postgenomic methods, and a n- ber of key perspectives chapters. Part I of Volume 1, Platelets and Megakaryocytes: Functional Assays, describes many well-established approaches to the study of platelet function, including aggregometry, secretion, arachidonic acid metabolism, procoagulant responses, pla- let adhesion under static or flow conditions, flow cytometry, and production of microparticles. Although one would ideally wish to perform experiments with human platelets, studies within the circulation using intravital microscopy require the use of animal models, which are described in Chapter 16, vol. 1. Part I. PerspectivesSignaling Receptors on Platelets and MegakaryocytesDonna Woulfe, Jing Yang, Nicolas Prevost, Peter O'Brien, Ryan Fortna, Massimiliano Tognolini, Hong Jiang, Jie Wu, and Lawrence F. BrassManipulation of Gene Expression in MegakaryocytesNikla R. Emambokus, George J. Murphy, and Jonathan FramptonIn Vitro Changes of Platelet Parameters: Lessons From Blood BankingJose Rivera, Maria L. Lozano, and Vicente VicentePharmacological Approaches to Studying Platelet Function: An OverviewSusanna M. O. HouraniInhibitors of Cellular Signaling Targets: Designs and LimitationsChris J. Vlahos and Louis F. StancatoPart II Signaling Techniques. Ligand-Binding Assays for CollagenStephanie M. Jung and Masaaki MoroiUse of Radiolabeled 2-Methylthio-ADP to Study P2Y Receptors on Platelets and Cell LinesPierre Savi and Jean-Marc HerbertLigand-Binding Assays: FibrinogenDermot CoxTechniques for the Analysis of Proteins by SDS-Polyacrylamide Gel Electrophoresis and Western BlottingJonathan M. GibbinsStudy of Tyrosine Kinases and Protein Tyrosine PhosphorylationJonathan M. GibbinsProtein Tyrosine PhosphatasesMatthew L. Jones and Alastair W. PooleThe Study of Serine-Threonine KinasesGertie Gorter and Jan Willem AkkermanPhosphoinositides: Lipid Kinases and PhosphatasesBernard PayrastreIsolation and Analysis of Platelet Lipid RaftsCorie N. Shrimpton, Karine Gousset, Fern Tablin, and Jose A. LopezMeasurement and Manipulation of [Ca2+]i in Suspensions of Platelets and Cell CulturesPhilippe Ohlmann, Beatrice Hechler, Jean-Pierre Cazenave, and Christian GachetMeasurement and Manipulation of Intracellular Ca2+ in Single Platelets and MegakaryocytesMichael J. Mason and Martyn P.Mahaut-SmithPatch-Clamp Recordings of Electrophysiological Events in the Platelet and MegakaryocyteMartyn P. Mahaut-SmithEvaluation of Nitrotyrosine-Containing Proteins in Blood PlateletsKhalid M. Naseem, Rocio Riba, and Max TroxlerNitric Oxide Signaling in PlateletsSylvia Y. Low and K. Richard BruckdorferSnake Venom Toxins Affecting Platelet FunctionRobert K. Andrews, Elizabeth E. Gardiner, and Michael C. BerndtPeptide Synthesis in the Study of Collagen-Platelet InteractionsC. Graham Knight, Catherine M. Onley, and Richard W. FarndalePlatelet PermeabilizationRobert FlaumenhaftPart III. Molecular and Post-Genomic TechniquesUsing Retroviruses to Express Genes in Primary Megakaryocyte Lineage CellsMeenakshi Gaur, George J. Murphy, Jonathan Frampton, and Andrew D. LeavittUse of Antisense Oligonucleotide Technology to Investigate Signaling Pathways in MegakaryocytesHava Avraham, Shalom Avraham, and Radoslaw ZagozdzonGFP Fusion Proteins to Study Signaling in Live CellsSimon A. Walker, Gyles E. Cozier, and Peter J. CullenTwo-Dimensional Polyacrylamide Gel Electrophoresis for Platelet ProteomicsKatrin Marcus and Helmut E. MeyerPreparation of mRNA and cDNA Libraries From Platelets and MegakaryocytesBenjamin Z. S. Paul, Jiango Jin, and Satya P. KunapuliPlatelet Receptor Structures and PolymorphismsThomas J. Kunicki, Steven Head, and Daniel R. SalomonGene Array Technology and the Study of Platelets and MegakaryocytesLloyd T. Lam and Emery H. BresnickIndex