Prins And In Situ Pcr Protocols by Franck Pellestor , Springer

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Springer Prins And In Situ Pcr Protocols by Franck Pellestor

The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Protocols edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ._x000D_ _x000D_Part I. Basic Methodology_x000D_ _x000D_ New Rapid Multicolor PRINS Protocol_x000D_ Ju Yan, Macoura Gadji, Kada Krabchi, and Regen Drouin_x000D_ _x000D_ PRINS Combined With Peptide Nucleic Acid Labeling_x000D_ Franck Pellestor and Petra Paulasova_x000D_ _x000D_ PRINS Combined With Indirect Immunofluorescence_x000D_ Philippe Coullin_x000D_ _x000D_ PRINS for the Detection of Unique Sequences_x000D_ Stephen S. Wachtel and Avirachan T. Tharapel_x000D_ _x000D_ Single-Copy and Point Mutation Analysis (Rolling-Circle PRINS)_x000D_ Jorn Koch_x000D_ _x000D_ Analysis of Sperm Aneuploidy by PRINS_x000D_ Franck Pellestor, Brigitte Andreo, Jacques Puechberty, Genevieve Lefort, and Pierre Sarda_x000D_ _x000D_ In Situ PCR Protocols_x000D_ Omar Bagasra and Twaina Harris_x000D_ _x000D_ Part II. Research and Clinical Applications_x000D_ _x000D_ Detection and Sizing of Telomeric and Other Simple Repeats by Dideoxy-PRINS_x000D_ Jorn Koch_x000D_ _x000D_ PRINS Evaluation of Chromosome Instability in Mammalian Cells by Detection of Repetitive DNA Sequences in Micronuclei_x000D_ Antonella Russo_x000D_ _x000D_ PRINS for the Detection of Gene Deletions in Cancer_x000D_ Avirachan T. Tharapel and Stephen S. Wachtel_x000D_ _x000D_ Combined Fluorescence In Situ Hybridization and PRINS for the Analysis of the Dystrophin Gene_x000D_ Liborio Stuppia, Dario La Sala, and Caterina Cinti_x000D_ _x000D_ Brain Tissue Preparations for Chromosomal PRINS Labeling_x000D_ Ivan Y. Iourov, Svetlana G. Vorsanova, Franck Pellestor, and Yuri B. Yurov_x000D_ _x000D_ PRINS on Plant Chromosomes_x000D_ Jiri Macas, Alice Navratilova, Marie Kubalakova, and Jaroslav Dolezel_x000D_ _x000D_ Dual-Color PRINS for In Situ Detection of Fetal Cells in Maternal Blood_x000D_ Kada Krabchi, Macoura Gadji, Ju Yan, and Regen Drouin_x000D_ _x000D_ PRINS as an Efficient Tool for Aneuploidy Assessment in Human Oocytes and Preimplantation Embryos_x000D_ Franck Pellestor, Brigitte Andreo, Tal Anahory, and Samir Hamamah_x000D_ _x000D_ Microdissection With PCR In Situ_x000D_ Allen T. Christian and ChristineHara_x000D_ _x000D_ Application of Reverse Transcription In Situ PCR in Cancer Analysis_x000D_ Tamotsu Takeuchi, Yoshihiro Adachi, Hiroshi Sonobe, and Yuji Ohtsuki_x000D_ _x000D_ In Situ Reverse Transcription PCR on Plant Tissues_x000D_ Zbigniew Przybecki, Ewa Siedlecka, Marcin Filipecki, and Ewa Urbanczyk-Wochniak_x000D_ _x000D_ In Situ PCR for the Detection of Human Cytomegalovirus in Suspension Cells During the Latent Phase of Infection_x000D_ Barry Slobedman_x000D_ _x000D_ Development and Adaptation of the PRINS Technology: An Overview_x000D_ Franck Pellestor_x000D_ _x000D_ Latest Developments in In Situ PCR_x000D_ Omar Bagasra and Twaina Harris_x000D_ _x000D_ Index_x000D_