Protein Targeting Protocols by Mark van der Giezen , Humana Press Inc.

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Humana Press Inc. Protein Targeting Protocols by Mark van der Giezen

In this updated volume, experts from around the world provide the latest protocols for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Emphasis is placed on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems. The book includes targeting protocols from different systems._x000D_ _x000D_ Contents_x000D_ _x000D_ Preface_x000D_ Contributors_x000D_ _x000D_ Bacterial systems_x000D_ 1 Localisation and mobility of bacterial proteins by confocal microscopy and fluorescence recovery after photobleaching (FRAP)_x000D_ Conrad Mullineaux_x000D_ 2 Membrane protein insertion and secretion in bacteria_x000D_ Jeanine de Keyzer, Martin van der Laan and Arnold J.M. Driessen_x000D_ 3 Identification of autotransporter proteins secreted by type V secretion systems in Gram-negative bacteria_x000D_ Yihfen T. Yen and Christos Stathopoulos_x000D_ 4 Periplasmic targeting of recombinant proteins in Escherichia coli _x000D_ Filipe J. M. Mergulhao and Gabriel A. Monteiro_x000D_ 5 In vitro analysis of the bacterial twin-arginine-dependent protein export_x000D_ Michael Moser, Sascha Panahandeh, Eva Holzapfel and Matthias Muller_x000D_ _x000D_ Eukaryotic systems_x000D_ 6 Nuclear import properties of the sex determining factor SRY_x000D_ Jade K. Forwood, Gurpreet Kaur and David A. Jans_x000D_ 7 The mitochondrial machinery for import of precursor protein, a review._x000D_ Kip Gabriel and Nikolaus Pfanner_x000D_ 8 A genetic method to identify mitochondrial proteins in living mammalian cells_x000D_ Takeaki Ozawa and Yoshio Umezawa_x000D_ 9 In vitro and in vivo methods to study protein import into plant mitochondria_x000D_ Shashi Bhushan, Pavel F. Pavlov, Charlotta Rudhe and Elzbieta Glaser_x000D_ 10 Protein targeting to mitochondria of Saccharomyces cerevisiae and Neurospora crassa; in vitro and in vivo studies_x000D_ Panagiotis Paptheodorou, Gra_nya Doma_ska and Joachim Rassow_x000D_ 11 A mitosome purification protocol based on Percoll density gradients and its use in validating the mitosomal nature of Entamoeba histolytica mitochondrial Hsp70_x000D_ Jorge Tovar, Sian S.E. Cox and Mark van der Giezen_x000D_ 12 The chloroplast protein import machinery, a review_x000D_ Friederike Hoermann, Jurgen Soll and Bettina Boelter_x000D_ 13 Import of plastid precursor proteins into pea chloroplasts_x000D_ Jurgen Soll and Bettina Boelter_x000D_ 14Protein targeting in 'secondary' or 'complex' chloroplasts_x000D_ Balbir K. Chaal and Beverley R. Green_x000D_ 15 Protein trafficking to the complex chloroplasts of Euglena_x000D_ Rostislav Vacula, Silvia Slavikova and Steven D. Schwartzbach_x000D_ 16 Fluorescent protein fusions for protein localization in plants_x000D_ John Runions, Chris Hawes and Smita Kurup_x000D_ 17 Genetic transformation; a tool to study protein targeting in diatoms_x000D_ Peter Kroth_x000D_ 18 Identification of proteins targeted into the endoplasmic reticulum by cDNA library screening_x000D_ Takeaki Ozawa and Yoshio Umezawa_x000D_ 19 Trafficking through the early secretory pathway of mammalian cells_x000D_ Theresa H. Ward_x000D_ 20 Studying protein export from the endoplasmic reticulum in plants_x000D_ Sally L. Hanton, Loren A. Matheson and Federica Brandizzi_x000D_ 21 Imaging the Golgi apparatus in living mitotic cells_x000D_ Nihal Altan-Bonnet_x000D_ 22 Evaluating yeast biosynthetic vacuolar transport_x000D_ Brian A. Davies, Darren S. carney and Bruce F. Horazdovsky_x000D_ 23 Targeting to lysosomes in mammalian cells: the biosynthetic and endocytic pathways_x000D_ Ann H. Erickson and Jeffrey P. Bocock_x000D_ 24 Monitoring autophagy in yeast: the Pho8_60 assay_x000D_ Daniel J. Klionsky_x000D_ 25 Protein targeting to yeast peroxisomes_x000D_ Ida van der Klei and Marten Veenhuis_x000D_ 26 Expression, localization and topology of fluorescently tagged plasma membrane-targeted transmembrane proteins_x000D_ Jeanne Shepshelovich and Koty Hirschberg _x000D_ _x000D_ General methods_x000D_ 27 Intracellular protein localization by immunoelectron microscopy_x000D_ Tetsuaki Osafune and Steven D. Schwartzbach_x000D_ 28 Separation of proteins by blue native electrophoresis (BN-PAGE)_x000D_ Olga Randelj, Joachim Rassow and Christian Motz_x000D_ 29 Computational prediction of subcellular localization_x000D_ Kenta Nakai and Paul Horton_x000D_ 30 Phylogenetic analysis to uncover organellar origins of nuclear-encoded genes_x000D_ Bernardo Foth_x000D_