Description
Humana Press Proteinligand Interactions Methods And Applications by G. Ulrich Nienhaus
A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.show more Isothermal Titration CalorimetryEdwin A. Lewis and Kenneth P. MurphyDirect Optical Detection of Protein-Ligand InteractionsFrank Gesellchen, Bastian Zimmermann, and Friedrich W. HerbergLabel-Free Detection of Protein-Ligand Interactions by the Quartz Crystal MicrobalanceAndreas Janshoff and Claudia SteinemMeasurement of Solvent Accessibility at Protein-Protein InterfacesJeffrey G. Mandell, Abel Baerga-Ortiz, Arnold M. Falick, and Elizabeth A. KomivesHydrophobic Interaction Chromatography: Harnessing Multivalent Protein-Surface Interactions for Purification ProceduresHerbert P. JennissenSedimentation Velocity Method in the Analytical Ultracentrifuge for the Study of Protein-Protein InteractionsClaus Urbanke, Gregor Witte, and Ute CurthProtein-Ligand Interaction Probed by Time-Resolved CrystallographyMarius Schmidt, Hyotcherl Ihee, Reinhard Pahl, and Vukica SrajerX-Ray Crystallography of Protein-Ligand InteractionsIlme SchlichtingCombined Use of XAFS and Crystallography for Studying Protein-Ligand Interactions in MetalloproteinsRichard W. Strange and S. Samar HasnainNMR Studies of Protein-Ligand InteractionsTill MaurerProbing Heme Protein-Ligand Interactions by UV/Visible Absorption SpectroscopyKarin Nienhaus and G. Ulrich NienhausUltrafast Time-Resolved IR Studies of Protein-Ligand InteractionsManho Lim and Philip A. AnfinrudMonitoring Protein-Ligand Interactions by Time-Resolved FTIR Difference SpectroscopyCarsten Koetting and Klaus GerwertProteins in Motion: Resonance Raman Spectroscopy as a Probe of Functional IntermediatesUri Samuni and Joel M. FriedmanFluorescence Polarization/Anisotropy Approaches to Study Protein-Ligand Interactions: Effects of Errors and UncertaintiesDavid M. Jameson and GaborMoczLigand Binding With Stopped-Flow Rapid MixingMark S. HargroveCircular Dichroism Spectroscopy for the Study of Protein-Ligand InteractionsAlison Rodger, Rachel Marrington, David Roper, and Stuart WindsorHigh-Throughput Screening of Interactions Between G Protein-Coupled Receptors and Ligands Using Confocal Optics MicroscopyLenka Zemanova, Andreas Schenk, Martin J. Valler, G. Ulrich Nienhaus, and Ralf HeilkerSingle-Molecule Study of Protein-Protein and Protein-DNA Interaction DynamicsH. Peter LuApplication of Fluorescence Correlation Spectroscopy to Hapten-Antibody BindingTheodore L. Hazlett, Qiaoqiao Ruan, and Sergey Y. TetinAtomic Force Microscopy Measurements of Protein-Ligand Interactions on Living CellsRobert H. Eibl and Vincent T. MoyComputer Simulation of Protein-Ligand Interactions: Challenges and ApplicationsSergio A. Hassan, Luis Gracia, Geetha Vasudevan, and Peter J. SteinbachForce Probe Molecular Dynamics SimulationsHelmut GrubmullerStudy of Ligand-Protein Interactions by Means of Density Functional Theory and First-Principles Molecular DynamicsCarme RoviraIndex